open access

https://hdl.handle.net/2115/44112

Adhesion, Spreading, and Proliferation of Endothelial Cells on Charged Hydrogels

英語

Tissue engineering

2009-11

Taylor & Francis

Journal of Adhesion

85(11)

839-868

As soft and wet scaffolds, hydrogels are attractive materials for tissue engineering due to their similarity in structure and properties to living tissue. For designing hydrogels as potential artificial tissues, some basic requirements, such as a high level of cellular viability, suitable viscoelasticity, and high mechanical strength are required. However, it is difficult to develop a hydrogel that satisfies even two of these requirements at the same time. In this review, our recent advances in developing synthetic hydrogels as cell culture scaffold are summarized. We found that endothelial cells (ECs) can proliferate directly on some synthetic hydrogels with negative charge, so long as the hydrogels have a Zeta potential lower than c.a. -20mV, and the cell behavior can be controlled by adjusting the hydrogel's charge density. Furthermore, confluent EC monolayers cultured on the hydrogels show excellent platelet compatibility, compared to EC monolayer cultured on a polystyrene plate. On the basis of the above study, we have further developed micro-patterned hydrogels for selective cell spreading, proliferation, and orientation. We have also developed tough hydrogels on which cells show viability. These results will promote the potential applications of synthetic hydrogels in tissue engineering.

学術雑誌論文

Accepted Manuscript

This is an electronic version of an article published in The Journal of Adhesion, 85(11), Nov. 2009, pp.839-868. The Journal of Adhesion is available online at: http://www.informaworld.com/openurl?genre=article&issn=1545-5823&volume=85&issue=11&spage=839

0021-8464