Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx

Miyashita, Tomomi; Ohashi, Takafumi; Shibata, Fukashi; Araki, Hajime; Hoshino, Yoichiro

Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx

Miyashita, Tomomi; Ohashi, Takafumi; Shibata, Fukashi et al.

Plant Cell, Tissue and Organ Culture, 2009, 98(3), 291-301

Permalink : https://hdl.handle.net/2115/44168

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アクセス権	open access
URI	https://hdl.handle.net/2115/44168
タイトル	Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx
著者	Miyashita, Tomomi ; Ohashi, Takafumi ; Shibata, Fukashi ; Araki, Hajime ; Hoshino, Yoichiro
著者名 Miyashita, Tomomi
著者名 Ohashi, Takafumi
著者名 Shibata, Fukashi
e-Rad_Researcher 30183148 著者名 Araki, Hajime
著者名 Hoshino, Yoichiro
言語	英語
キーワード	Aneuploid
	Endosperm culture
	Hexaploid
	Lonicera caerulea
	Ploidy level
	Regeneration
発行日	2009-09
出版者	Springer Netherlands
収録物名	Plant Cell, Tissue and Organ Culture
巻(号)	98(3)
ページ	291-301
抄録	An endosperm culture of Haskap (Lonicera caerulea var. emphyllocalyx) was established to develop polyploid plants and investigate the regeneration ability of the endosperm. Based on histological analysis of embryo and endosperm development, endosperms at the globular to early torpedo-stages of developing embryos were used to initiate an endosperm culture. Formation of shoot primordia was observed on Murashige and Skoog (MS) medium (1962) containing benzyladenine (BA) and indole-3-butyric acid (IBA). Shoot primordium formation was confirmed in some genotypes with regeneration frequencies ranging between 1.9% to 10.0%. These proliferated on 1/2 MS medium containing 2.89 μM gibberellic acid (GA3), and then elongated and rooted on MS medium containing 0.44 μM BA and 2.89 μM GA3. These shoots developed into plantlets on 1/2 MS medium. Plantlets maintained ploidy of the endosperm following flow cytometric analysis, thus confirming that these were derived from the endosperm. These results indicated that endosperms were capable of regeneration.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0167-6857
EISSN	1573-5044
NCID	AA10472996
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11240-009-9562-6