Chromosome banding and FISH with rDNA in diploid and tetraploid loach, Misgurnus anguillicaudatus

Li, Ya-Juan; Tian, Yi; Zhang, Ming-Zhao et al.

Ichthyological Research, 2010, 57(4), 358-366

Permalink : https://hdl.handle.net/2115/44223

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アクセス権
open access
URI
https://hdl.handle.net/2115/44223
タイトル
Chromosome banding and FISH with rDNA in diploid and tetraploid loach, Misgurnus anguillicaudatus
著者
Li, Ya-Juan ; Tian, Yi ; Zhang, Ming-Zhao ; Tian, Ping-Ping ; Yu, Zhou ; Abe, Syuiti ; Arai, Katsutoshi
著者名
Li, Ya-Juan
著者名
Tian, Yi
著者名
Zhang, Ming-Zhao
著者名
Tian, Ping-Ping
著者名
Yu, Zhou
著者名
Abe, Syuiti
e-Rad_Researcher
00137902
著者名
Arai, Katsutoshi
言語
英語
キーワード
Ag-NORs
Cobitidae
Fluorescence in-situ hybridization
Polyploidy
Ribosomal genes
Weatherfish
発行日
2010
出版者
Springer
収録物名
Ichthyological Research
巻(号)
57(4)
ページ
358-366
抄録
The chromosomes of diploid and tetraploid loach, Misgurnus anguillicaudatus, were analyzed by staining with Ag, Chromomycin A3 (CMA3)/ Distamycin A (DA), and DA/4',6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. Nucleolus organizing regions (NORs) were mapped to the telomeric region of the short arms of the largest (first) metacentric chromosome pair in diploid loach with 2n=50 and the homologous quartet in tetraploid loach with 4n=100. The NORs were positive at the same region of the first metacentric chromosome for Ag- and CMA3/DA-stainings, but negative for DA/DAPI-staining. Four signals at the homologues within the same quartet suggest the duplication of entire genome from diploid to tetraploid status. However, size difference was detected among rDNA signals by FISH and CMA3-banding.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The original publication is available at www.springerlink.com
PISSN
1341-8998
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s10228-010-0168-0