Perilipin Overexpression in White Adipose Tissue Induces a Brown Fat-Like Phenotype

Sawada, Takashi; Miyoshi, Hideaki; Shimada, Kohei; Suzuki, Akira; Okamatsu-Ogura, Yuko; Perfield, James W., II; Kondo, Takuma; Nagai, So; Shimizu, Chikara; Yoshioka, Narihito; Greenberg, Andrew S.; Kimura, Kazuhiro; Koike, Takao

Perilipin Overexpression in White Adipose Tissue Induces a Brown Fat-Like Phenotype

Sawada, Takashi; Miyoshi, Hideaki; Shimada, Kohei et al.

PLoS One, 2010, 5(11), e14006

Permalink : https://hdl.handle.net/2115/44393

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アクセス権	open access
URI	https://hdl.handle.net/2115/44393
タイトル	Perilipin Overexpression in White Adipose Tissue Induces a Brown Fat-Like Phenotype
著者	Sawada, Takashi ; Miyoshi, Hideaki ; Shimada, Kohei ; Suzuki, Akira ; Okamatsu-Ogura, Yuko ; Perfield, James W., II ; Kondo, Takuma ; Nagai, So ; Shimizu, Chikara ; Yoshioka, Narihito ; Greenberg, Andrew S. ; Kimura, Kazuhiro ; Koike, Takao
著者名 Sawada, Takashi
e-Rad_Researcher 30360902 著者名 Miyoshi, Hideaki
著者名 Shimada, Kohei
著者名 Suzuki, Akira
e-Rad_Researcher 90527178 著者名 Okamatsu-Ogura, Yuko
著者名 Perfield, James W., II
著者名 Kondo, Takuma
e-Rad_Researcher 70507590 著者名 Nagai, So
e-Rad_Researcher 00292029 著者名 Shimizu, Chikara
e-Rad_Researcher 20200941 著者名 Yoshioka, Narihito
著者名 Greenberg, Andrew S.
e-Rad_Researcher 30192561 著者名 Kimura, Kazuhiro
e-Rad_Researcher 80146795 著者名 Koike, Takao
言語	英語
発行日	2010-11-16
出版者	Public Library of Science
収録物名	PLoS One
巻(号)	5(11)
ページ	e14006
抄録	Background: Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Previously, we reported that adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype, we performed additional studies in this transgenic mouse model. Methodology and Principal Findings: When compared to control animals, whole body energy expenditure was increased in the transgenic mice. Subsequently, we performed DNA microarray analysis and real-time PCR on white adipose tissue. Consistent with the metabolic chamber data, we observed increased expression of genes associated with fatty acid β-oxidation and heat production, and a decrease in the genes associated with lipid synthesis. Gene expression of Pgc1a, a regulator of fatty acid oxidation and Ucp1, a brown adipocyte specific protein, was increased in the white adipose tissue of the transgenic mice. This observation was subsequently verified by both Western blotting and histological examination. Expression of RIP140, a regulator of white adipocyte differentiation, and the lipid droplet protein FSP27 was decreased in the transgenic mice. Importantly, FSP27 has been shown to control gene expression of these crucial metabolic regulators. Overexpression of PeriA in 3T3-L1 adipocytes also reduced FSP27 expression and diminished lipid droplet size. Conclusions: These findings demonstrate that overexpression of PeriA in white adipocytes reduces lipid droplet size by decreasing FSP27 expression and thereby inducing a brown adipose tissue-like phenotype. Our data suggest that modulation of lipid droplet proteins in white adipocytes is a potential therapeutic strategy for the treatment of obesity and its related disorders.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 2.5 Generic
PISSN	1932-6203
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0014006