EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae

Ndindeng, Sali Atanga; Miki, Shinsuke; Abe, Ayumi et al.

Journal of General Plant Pathology, 2010, 76(6), 377-381

Permalink : https://hdl.handle.net/2115/44584

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アクセス権
open access
URI
https://hdl.handle.net/2115/44584
タイトル
EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae
著者
Ndindeng, Sali Atanga ; Miki, Shinsuke ; Abe, Ayumi ; Asano, Kozo ; Sone, Teruo
著者名
Ndindeng, Sali Atanga
著者名
Miki, Shinsuke
著者名
Abe, Ayumi
著者名
Asano, Kozo
著者名
Sone, Teruo
言語
英語
キーワード
Appressorium
DNA double-strand breaks
Rice colonization
Hyphal growth
Magnaporthe oryzae
Rice blast disease
発行日
2010-12
出版者
Springer Japan
収録物名
Journal of General Plant Pathology
巻(号)
76(6)
ページ
377-381
抄録
In order to detect and enumerate DNA double-strand breaks (DSBs) during the life cycle of Magnaporthe oryzae, an expression vector for GFP-Rhm51 fusion protein was constructed and introduced into the strain Ina86-137. Rhm51-GFP foci were detected and the number of foci in mitomycin-C-treated mycelia was higher than in untreated samples, indicating that the foci visualized DSBs occurred during the life cycle. Rhm51-GFP foci were observed in all stages of the asexual life cycle including the invasive hyphae formed in an intact rice leaf sheath, demonstrating that M. oryzae suffers DSBs during vegetative and infective growth.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The final publication is available at www.springerlink.com
PISSN
1345-2630
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1007/s10327-010-0271-0