open access

https://hdl.handle.net/2115/44840

Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety : Effect of elasticity of hydrogel

英語

platelet adhesion

2010-08

WILEY

Journal of Biomedical Materials Research Part A

95A(2)

531-542

We measured the gene expression, glycocalyx content, and surface properties of human coronary artery endothelial cells (HCAECs) cultured on poly(sodium p-styrene sulfonate) (PNaSS) hydrogels with various levels of elasticity ranged in 3 kPa ~ 300 kPa. We found that all HCAECs reached confluence on these hydrogels while retaining the similar expression of EC-specific markers to that on polystyrene (PS), a widely used scaffold in cell culture in vitro. Real-time polymerase chain reaction (PCR) and glycosaminoglycan (GAG) assay showed that the amount of EC-specific glycocalyx secreted by HCAECs cultured on PNaSS gels was higher than that cultured on PS, and it increased with an increase of gel elasticity. Furthermore, the HCAECs cultured on PNaSS gels showed excellent property against platelet adhesion and lower surface friction than that on PS. The platelet adhesion and surface friction of HCAECs cultured on PNaSS gels also depend on the elasticity of gels. The largest amount of EC-specific glycocalyx, excellent blood compatibility, and the lowest friction were observed when the elastic modulus of the gel was larger than 60 kPa. Overall, HCAECs cultured on these hydrogels have better properties than those cultured on PS scaffolds, demonstrating the PNaSS gels can be used as potential tissue engineering material for blood vessels.

学術雑誌論文

Accepted Manuscript

This is the pre-peer-reviewed version of the following article: FULL CITE, which has been published in final form at [Journal of Biomedical Materials Research Part A：Aug-2010, 95A(2), pp.531-542.]

1549-3296

1552-4965