Isolation and identification of new vasodilative substances in diesel exhaust particles

Seki, Koh-ichi; Noya, Yoichi; Mikami, Yusuke; Taneda, Shinji; Suzuki, Akira K.; Kuge, Yuji; Ohkura, Kazue

Isolation and identification of new vasodilative substances in diesel exhaust particles

Seki, Koh-ichi; Noya, Yoichi; Mikami, Yusuke et al.

Environmental Science and Pollution Research, 2010, 17(3), 717-723

Permalink : https://hdl.handle.net/2115/44897

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アクセス権	open access
URI	https://hdl.handle.net/2115/44897
タイトル	Isolation and identification of new vasodilative substances in diesel exhaust particles
著者	Seki, Koh-ichi ; Noya, Yoichi ; Mikami, Yusuke ; Taneda, Shinji ; Suzuki, Akira K. ; Kuge, Yuji ; Ohkura, Kazue
著者名 Seki, Koh-ichi
著者名 Noya, Yoichi
著者名 Mikami, Yusuke
著者名 Taneda, Shinji
著者名 Suzuki, Akira K.
著者名 Kuge, Yuji
著者名 Ohkura, Kazue
言語	英語
キーワード	Acidic components
	Diesel exhaust particles
	Fractionation
	3-Hydroxy-4-nitrobenzoic acid
	4-Hydroxy-3-nitrobenzoic acid
	Separation method
	Vasodilatory compounds
発行日	2010-03
出版者	Springer Berlin / Heidelberg
収録物名	Environmental Science and Pollution Research
巻(号)	17(3)
ページ	717-723
抄録	Goal, Scope, and Background: We recently developed a new isolation method for diesel exhaust particles (DEP), involving successive extraction with H2O, sodium bicarbonate, and sodium hydroxide, in which the sodium hydroxide extract was found to consist of phenolic components. Analysis of the extract revealed that vasodilative active nitrophenols are in DEP in significantly higher concentration than those estimated by an earlier method involving a combination of solvent extraction and repeated chromatography. These findings indicated that our new procedure offers a simple, efficient and reliable method for the isolation and identification of bioactive substances in DEP. This encouraged us to extend our work to investigating new vasodilatory substances in the sodium bicarbonate extract. Materials and Methods: DEP were collected from the exhaust of a 4JB1-type engine (ISUZU Automobile Co., Tokyo, Japan). GC-MS analysis was performed with a GCMS-QP2010 instrument (Shimadzu, Kyoto, Japan). Results: DEP dissolved in 1-butanol was successively extracted with water, sodium bicarbonate, and then aqueous sodium hydroxide. The sodium bicarbonate extract was neutralized and the resulting mixture of acidic components was subjected to reverse-phase (RP) column chromatography followed by RP-HPLC with fractions assayed for vasodilative activity. This led to the identification of terephthalic acid, p-hydroxybenzoic acid, isophthalic acid, phthalic acid, 3-hydroxy-4-nitrobenzoic acid, 4-hydroxy-3-nitrophenol, and 1,4,5-naphthalene tricarboxylic acid as components of DEP. Discussion: The sodium bicarbonate extract was rich in the acidic components. Repeated reverse-phase chromatography resulted in the successful isolation of several acidic substances including the new vasodilative materials, 4-hydroxy-3-nitrobenzoic acid, and 3-hydroxy-4-nitrobenzoic acid. Conclusion: Our new fractionation method for DEP has made possible the isolation of new vasodilative compounds from the sodium bicarbonate extract.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0944-1344
関連情報	PMID 19557451
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11356-009-0207-4