In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2

Kashiwazaki, Haruhiko; Yamaguchi, Keisuke; Harada, Naoki; Akazawa, Toshiyuki; Murata, Masaru; Iizuka, Tadashi; Ikoma, Toshiyuki; Tanaka, Junzo; Inoue, Nobuo

In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2

Kashiwazaki, Haruhiko; Yamaguchi, Keisuke; Harada, Naoki et al.

Journal of Hard Tissue Biology, 2010, 19(3), 181-186

Permalink : https://hdl.handle.net/2115/44925

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アクセス権	open access
URI	https://hdl.handle.net/2115/44925
タイトル	In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2
著者	Kashiwazaki, Haruhiko ; Yamaguchi, Keisuke ; Harada, Naoki ; Akazawa, Toshiyuki ; Murata, Masaru ; Iizuka, Tadashi ; Ikoma, Toshiyuki ; Tanaka, Junzo ; Inoue, Nobuo
著者名 Kashiwazaki, Haruhiko
著者名 Yamaguchi, Keisuke
著者名 Harada, Naoki
著者名 Akazawa, Toshiyuki
著者名 Murata, Masaru
著者名 Iizuka, Tadashi
著者名 Ikoma, Toshiyuki
著者名 Tanaka, Junzo
著者名 Inoue, Nobuo
言語	英語
キーワード	Chitosan
	Hydroxyapatite (HAp)
	Osteoinduction
	Bioabsorption
	Recombinant human bone morphogenetic protein-2 (rhBMP-2)
発行日	2010-12
出版者	硬組織再生生物学会
収録物名	Journal of Hard Tissue Biology
巻(号)	19(3)
ページ	181-186
抄録	We developed a porous chitosan/hydroxyapatite (HAp) composite, in which the HAp nanocrystals align along the chitosan molecules, and examined the biocompatibility, osteoinductive activity, and the ability to act as a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) of this novel biomaterial. The composite was subcutaneously implanted into the backs of 11-week-old SD rats, with or without rhBMP-2 (5μg). At 2 and 8 weeks after the implantation, the composite was explanted for morphohistological evaluation. In the presence of rhBMP-2, ectopic bone formation was found at 2 weeks and maturation of the newly formed bone around the composite at 8 weeks. Chitosan/HAp composite alone caused little inflammation, and new blood vessel growth and multinucleated giant cells were found around the composite, accompanied with roughening of the surface due to degradation at 2 weeks; however, neither cartilage nor bone formation was found around the composite. With rhBMP-2, the bioabsorption of the composite was accelerated as the rhBMP-2-induced bone matured. Histomorphometrical analysis showed that the mean value of the composite areas with rhBMP-2 was significantly smaller than that without rhBMT-2 at 2 and 8 weeks after the implantation. These results suggested that the novel chitosan/HAp composite was an effective bioabsorbable material as a carrier of rhBMP-2.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1341-7649
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2485/jhtb.19.181