Detection of all known filovirus species by reverse transcription-polymerase chain reaction using a primer set specific for the viral nucleoprotein gene

Ogawa, Hirohito; Miyamoto, Hiroko; Ebihara, Hideki; Ito, Kimihito; Morikawa, Shigeru; Feldmann, Heinz; Takada, Ayato

Detection of all known filovirus species by reverse transcription-polymerase chain reaction using a primer set specific for the viral nucleoprotein gene

Ogawa, Hirohito; Miyamoto, Hiroko; Ebihara, Hideki et al.

Journal of Virological Methods, 2011, 171(1), 310-313

Permalink : https://hdl.handle.net/2115/44979

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/44979
タイトル	Detection of all known filovirus species by reverse transcription-polymerase chain reaction using a primer set specific for the viral nucleoprotein gene
著者	Ogawa, Hirohito ; Miyamoto, Hiroko ; Ebihara, Hideki ; Ito, Kimihito ; Morikawa, Shigeru ; Feldmann, Heinz ; Takada, Ayato
著者名 Ogawa, Hirohito
著者名 Miyamoto, Hiroko
著者名 Ebihara, Hideki
e-Rad_Researcher 60396314 著者名 Ito, Kimihito
e-Rad_Researcher 00167686 著者名 Morikawa, Shigeru
著者名 Feldmann, Heinz
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
キーワード	Filovirus
	Marburg virus
	Ebola virus
	Diagnosis
	RT-PCR
発行日	2011-01
出版者	Elsevier B.V.
収録物名	Journal of Virological Methods
巻(号)	171(1)
ページ	310-313
抄録	The filoviruses, Marburg virus (MARV) and Ebola virus (EBOV), are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates. Sporadic outbreaks of filovirus infection have occurred in Central Africa and parts of Asia. Identification of the natural reservoir animals that are unknown yet and epidemiological investigations are current challenges to forestall outbreaks of filovirus diseases. The filovirus species identified currently include one in the MARV group and five in the EBOV group, with large genetic variations found among the species. Therefore, it has been difficult to develop a single sensitive assay to detect all filovirus species, which would advance laboratory diagnosis greatly in endemic areas. In this study, a highly sensitive universal RT-PCR assay targeting the nucleoprotein (NP) gene of filoviruses was developed. The genomic RNAs of all known MARV and EBOV species were detected by using an NP-specific primer set. In addition, this RT-PCR procedure was verified further for its application to detect viral RNAs in tissue samples of animals infected experimentally and blood specimens of infected patients. This assay will be a useful method for diagnostics and epidemiological studies of filovirus infections.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0166-0934
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jviromet.2010.11.010