Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake

Furukawa, Ryo; Yamada, Yuma; Takenaga, Mitsuko; Igarashi, Rie; Harashima, Hideyoshi

Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake

Furukawa, Ryo; Yamada, Yuma; Takenaga, Mitsuko et al.

Biochemical and Biophysical Research Communications, 2011, 404(3), 796-801

Permalink : https://hdl.handle.net/2115/44983

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アクセス権	open access
URI	https://hdl.handle.net/2115/44983
タイトル	Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake
著者	Furukawa, Ryo ; Yamada, Yuma ; Takenaga, Mitsuko ; Igarashi, Rie ; Harashima, Hideyoshi
著者名 Furukawa, Ryo
著者名 Yamada, Yuma
著者名 Takenaga, Mitsuko
著者名 Igarashi, Rie
著者名 Harashima, Hideyoshi
言語	英語
キーワード	Lecithinized superoxide dismutase (PC-SOD)
	Octaarginine-modified liposomes
	Anti-oxidant therapy
	Reactive oxygen species (ROS)
	Mitochondria
発行日	2011-01-21
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	404(3)
ページ	796-801
抄録	The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane (R. Igarashi et al, J. Pharmacol. Exp. Ther. 271 (1994) 1672-7). Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study (I.A. Khalil et al. J. Biol. Chem. 281 (2006) 3544-51). Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O-2^[-]) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 21168389
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2010.12.062