Deficiency of macrophage migration inhibitory factor gene delays healing of the medial collateral ligament : A biomechanical and biological study

Kitayama, Soichiro; Onodera, Shin; Kondo, Eiji; Kobayashi, Takumi; Miyatake, Shin; Kitamura, Nobuto; Tohyama, Harukazu; Yasuda, Kazunori

Deficiency of macrophage migration inhibitory factor gene delays healing of the medial collateral ligament : A biomechanical and biological study

Kitayama, Soichiro; Onodera, Shin; Kondo, Eiji et al.

Journal of Biomechanics, 2011, 44(3), 494-500

Permalink : https://hdl.handle.net/2115/45022

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アクセス権	open access
URI	https://hdl.handle.net/2115/45022
タイトル	Deficiency of macrophage migration inhibitory factor gene delays healing of the medial collateral ligament : A biomechanical and biological study
著者	Kitayama, Soichiro ; Onodera, Shin ; Kondo, Eiji ; Kobayashi, Takumi ; Miyatake, Shin ; Kitamura, Nobuto ; Tohyama, Harukazu ; Yasuda, Kazunori
著者名 Kitayama, Soichiro
著者名 Onodera, Shin
著者名 Kondo, Eiji
著者名 Kobayashi, Takumi
著者名 Miyatake, Shin
著者名 Kitamura, Nobuto
著者名 Tohyama, Harukazu
著者名 Yasuda, Kazunori
言語	英語
キーワード	Medial collateral ligament
	Macrophage migration inhibitory factor
	Healing
	Mouse
	Matrix metalloproteinase
発行日	2011-02-03
出版者	Elsevier
収録物名	Journal of Biomechanics
巻(号)	44(3)
ページ	494-500
抄録	The role played by macrophage migration inhibitory factor (MIF) in the process of wound healing is controversial. Besides, there have been no reports that investigated the expression or the role of MIF in the repair process after ligament injury. In this study, we hypothesized that the deficiency in MIF gene might delay ligament healing in mice. The aim of this study was to clarify this hypothesis using MIF gene-deficient mice (MIFKO) and murine model of injury to the medial collateral ligament (MCL). Biomechanical testing showed that the levels of mechanical properties were significantly lower in MIFKO than in wild-type mice (WT) on day 28 after injury. Levels of matrix metalloproteinase (MMP)-2 and -13 mRNA in the healing tissue were significantly lower in MIFKO than in WT on day 28 and on day 7, respectively. Histologically, healing tissues in MIFKO exhibited prolonged hypertrophy, poor vascularity, and prolonged increase in cell number compared with those in WT. Taken together, it was suggested that MIFKO exhibited delayed healing of the MCL, which might be caused by lower mRNA expression of MMP-2 and -13.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0021-9290
関連情報	PMID 20950810
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jbiomech.2010.09.018