Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5

Yoshii, Kentaro; Igarashi, Manabu; Ito, Kimihito; Kariwa, Hiroaki; Holbrook, Michael R.; Takashima, Ikuo

Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5

Yoshii, Kentaro; Igarashi, Manabu; Ito, Kimihito et al.

Virus Research, 2011, 155(1), 61-68

Permalink : https://hdl.handle.net/2115/45025

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/45025
タイトル	Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5
著者	Yoshii, Kentaro ; Igarashi, Manabu ; Ito, Kimihito ; Kariwa, Hiroaki ; Holbrook, Michael R. ; Takashima, Ikuo
e-Rad_Researcher 50421988 著者名 Yoshii, Kentaro
e-Rad_Researcher 10374240 著者名 Igarashi, Manabu
e-Rad_Researcher 60396314 著者名 Ito, Kimihito
e-Rad_Researcher 70224714 著者名 Kariwa, Hiroaki
著者名 Holbrook, Michael R.
e-Rad_Researcher 30002083 著者名 Takashima, Ikuo
言語	英語
キーワード	Omsk hemorrhagic fever
	Flavivirus
	Infectious cDNA
	Viral replication
発行日	2011-01
出版者	Elsevier B.V.
収録物名	Virus Research
巻(号)	155(1)
ページ	61-68
抄録	Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis serocomplex of flaviviruses, and causes hemorrhagic disease in humans. In this study, an infectious cDNA of OHFV was constructed to investigate the molecular mechanisms involved in OHFV pathogenesis for the first time. Our cDNA clone was capable of producing infectious virus which is genetically identical to the parental Guriev strain, and the recombinant virus showed similar biological properties to the parental virus including growth kinetics and virulence characteristics. While characterizing the cDNAs, fortuitous mutations at NS2A position 46 and NS5 position 836 were found to affect viral production. By using a viral replicon expressing luciferase, it was shown that both of the mutations produced a defect in RNA replication and that the NS5 mutation induced a temperature-sensitive phenotype, indicating the importance of these residues in RNA replication. This infectious cDNA will be a useful tool to study the replication and pathogenesis of OHFV.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0168-1702
関連情報	PMID 20817056
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.virusres.2010.08.023