open access

https://hdl.handle.net/2115/45088

Genetic diversity and diet specificity of ruminal Prevotella revealed by 16S rRNA gene-based analysis

英語

16S rRNA gene

2010-04

Blackwell Publishing

FEMS Microbiology Letters

305(1)

49-57

16S rDNA-based analysis of rumen Prevotella was carried out to estimate the diversity and diet specificity of bacteria belonging to this genus. Total DNA was extracted from rumen digesta of three sheep fed two diets with different hay-to-concentrate ratios (10:1 and 1:2). Real-time PCR quantification of Prevotella revealed that the relative abundance of this genus in total rumen bacteria was up to 19.7%, while the representative species Prevotella bryantii and Prevotella ruminicola accounted for only 0.6% and 3.8%, respectively. Denaturing gradient gel electrophoresis analysis for Prevotella revealed shifts in community composition with diet. Analysis of 16S rDNA clone libraries showed significant differences (P=0.001) between clones detected from the sheep on the diets with different hay-to-concentrate ratios. The majority (87.8%) of Prevotella clones had < 97% sequence similarity with known rumen Prevotella. These data suggest that uncultured Prevotella is more abundant than known Prevotella and that members of this genus appear to have specific metabolic niches.

学術雑誌論文

Accepted Manuscript

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0378-1097