Failure of mycoplasma lipoprotein MALP-2 to induce NK cell activation through dendritic cell TLR2

Sawahata, Ryoko; Shime, Hiroaki; Yamazaki, Sayuri; Inoue, Norimitsu; Akazawa, Takashi; Fujimoto, Yukari; Fukase, Koichi; Matsumoto, Misako; Seya, Tsukasa

Failure of mycoplasma lipoprotein MALP-2 to induce NK cell activation through dendritic cell TLR2

Sawahata, Ryoko; Shime, Hiroaki; Yamazaki, Sayuri et al.

Microbes and Infection, 2011, 13(4), 350-358

Permalink : https://hdl.handle.net/2115/45255

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アクセス権	open access
URI	https://hdl.handle.net/2115/45255
タイトル	Failure of mycoplasma lipoprotein MALP-2 to induce NK cell activation through dendritic cell TLR2
著者	Sawahata, Ryoko ; Shime, Hiroaki ; Yamazaki, Sayuri ; Inoue, Norimitsu ; Akazawa, Takashi ; Fujimoto, Yukari ; Fukase, Koichi ; Matsumoto, Misako ; Seya, Tsukasa
著者名 Sawahata, Ryoko
著者名 Shime, Hiroaki
著者名 Yamazaki, Sayuri
著者名 Inoue, Norimitsu
著者名 Akazawa, Takashi
著者名 Fujimoto, Yukari
著者名 Fukase, Koichi
著者名 Matsumoto, Misako
著者名 Seya, Tsukasa
言語	英語
キーワード	Toll-like receptor 2
	MyD88
	Macrophage-activating lipopeptide 2
	Dendritic cells
	NK activation
発行日	2011-04
出版者	Elsevier Masson SAS
収録物名	Microbes and Infection
巻(号)	13(4)
ページ	350-358
抄録	Macrophage-activating lipopeptide 2 (MALP-2), a mycoplasmal diacylated lipopeptide with palmitic acid moiety (Pam2), activates Toll-like receptor (TLR) 2 to induce inflammatory cytokines. TLR2 is known to mature myeloid dendritic cells (mDC) to drive mDC contact-mediated natural killer (NK) cell activation. Here we tested if MALP-2 activates NK cells through stimulation of TLR2 on mDC. Although synthetic MALP-2 with 6 or 14 amino acids (a.a.) stretch (designated as s and f) matured mDC to induce IL-6, IL-12p40 and TNF-α to a similar extent, they far less activated NK cells than Pam2CSK4, a positive control of 6 a.a.-containing diacyl lipopeptide. MALP-2s and f were TLR2/6 agonists and activate the MyD88 pathway similar to Pam2CSK4, but MALP-2s having the CGNNDE sequence acted on mDC TLR2 to barely induce external NK activation. Even the s form, with slightly high induction of IL-6 compared to the f form, barely induced in vivo growth retardation of NK-sensitive implant tumor. Pam2CSK4 and MALP-2 have the common lipid moiety but different peptides, which are crucial for NK cell activation. The results infer that MALP-2 is applicable to a cytokine inducer but not to an adjuvant for antitumor NK immunotherapy.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1286-4579
関連情報	PMID 21172450
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.micinf.2010.12.003