Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage

Hashiguchi, Taishi; Kobayashi, Takanari; Fongmoon, Duriya; Shetty, Ajaya Kumar; Mizumoto, Shuji; Miyamoto, Nobuyuki; Nakamura, Toshikazu; Yamada, Shuhei; Sugahara, Kazuyuki

Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage

Hashiguchi, Taishi; Kobayashi, Takanari; Fongmoon, Duriya et al.

Biochimica et Biophysica Acta (BBA) : General Subjects, 2011, 1810(4), 406-413

Permalink : https://hdl.handle.net/2115/45301

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アクセス権	open access
URI	https://hdl.handle.net/2115/45301
タイトル	Demonstration of the hepatocyte growth factor signaling pathway in the in vitro neuritogenic activity of chondroitin sulfate from ray fish cartilage
著者	Hashiguchi, Taishi ; Kobayashi, Takanari ; Fongmoon, Duriya ; Shetty, Ajaya Kumar ; Mizumoto, Shuji ; Miyamoto, Nobuyuki ; Nakamura, Toshikazu ; Yamada, Shuhei ; Sugahara, Kazuyuki
著者名 Hashiguchi, Taishi
著者名 Kobayashi, Takanari
著者名 Fongmoon, Duriya
著者名 Shetty, Ajaya Kumar
著者名 Mizumoto, Shuji
著者名 Miyamoto, Nobuyuki
著者名 Nakamura, Toshikazu
著者名 Yamada, Shuhei
著者名 Sugahara, Kazuyuki
言語	英語
キーワード	Chondroitin sulfate
	Cartilage
	Neurite outgrowth-promoting activity
	Hepatocyte growth factor
	c-Met receptor
発行日	2011-04
出版者	Elsevier B.V.
収録物名	Biochimica et Biophysica Acta (BBA) : General Subjects
巻(号)	1810(4)
ページ	406-413
抄録	Chondroitin sulfate (CS) was isolated from ray fish cartilage, an industrial waste, after protease digestion, and its structure and neurite outgrowth-promoting (NOP) activity were analyzed to investigate a potential application to nerve regeneration. A disaccharide analysis using chondroitinase ABC revealed that the major unit in the CS preparation was GlcUA-GalNAc(6-O-sulfate) (63%), where GlcUA and GalNAc represent D-glucuronic acid and N-acetyl-D-galactosamine, respectively. Small proportions of other disaccharide units, GlcUA-GalNAc(4-O-sulfate) (25%), GlcUA(2-O-sulfate)-GalNAc(6-O-sulfate) (7%), and GlcUA-GalNAc (5%), were also detected. The average molecular mass of CS was estimated to be 142 kDa by gel-filtration chromatography. The prepration showed NOP activity in vitro, which was eliminated by digestion with chondroitinase ABC, suggesting that a polymeric structure is required for the activity. Antibodies against hepatocyte growth factor (HGF) and its receptor c-Met suppressed the NOP activity, suggesting the involvement of the HGF signaling pathway in the in vitro NOP activity of the CS preparation. Since the specific binding of HGF to the CS preparation was also demonstrated by surface plasmon resonance spectroscopy, the CS chains were fractionated using an HGF-immobilized column into unbound and bound fractions accounting for 44 and 56% of the total yield, respectively. The latter contained a higher proportion of the GlcUA(2-O-sulfate)-GalNAc(6-O-sulfate) unit, and showed greater NOP activity than the former, indicating that the HGF-binding domain contains GlcUA(2-O-sulfate)-GalNAc(6-O-sulfate) and is involved in the NOP activity. CS from ray cartilage may have potential pharmaceutical applications.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0304-4165
関連情報	PMID 21223992
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbagen.2011.01.001