open access

https://hdl.handle.net/2115/45422

Stabilizing effect of Ca2+ on myosin and myofibrils of squid mantle muscle as affected by heating conditions

英語

Ca2+

2011-05

Springer Japan

Fisheries Science

77(3)

425-430

Suppressive effect of Ca2+ on the thermal denaturation of myosin and on myofibrils of squid mantle muscle was compared. The stabilization effect on myosin was smaller than that on myofibrils, and was not affected by KCl concentration. The stabilizing effect of Ca2+ on myosin decreased with the lowering the temperature for heating showing no stabilization at 20℃, while the effect on myofibrils was evenly detected at all temperatures tested. Stabilizing effect of Ca2+ on myosin disappeared even at 30℃ in the presence of sorbitol showing a small inactivation rate, while the effect of Ca2+ was equally detected on myofibrils irrespective of the reduction in inactivation rate with sorbitol. Stabilization of myosin by Ca2+ again appeared even in the presence of sorbitol by raising the heating temperature to 38℃. It was suggested that Ca2+ confers stabilization on myosin only when myosin is in under unstable conditions. Stabilization effect of Ca2+ on myosin was enhanced upon F-actin binding, namely Ca2+ bound myosin was more significantly stabilized by F-actin binding, and the effect was no longer affected by the conditions for heating.

学術雑誌論文

Accepted Manuscript

© 2011 The Japanese Society of Fisheries Science

0919-9268