The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids

Sugihara, Shinji; Orikasa, Yoshitake; Okuyama, Hidetoshi

FEMS Microbiology Letters, 2010, 307(2), 207-211

Permalink : https://hdl.handle.net/2115/45533

このアイテムのアクセス数:1,257件（2026-03-15 05:14 集計）

閲覧可能ファイル 

ファイル フォーマット サイズ 閲覧回数 説明
FEMSML307-2_207-211 pdf 160 KB 1,216

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
open access
URI
https://hdl.handle.net/2115/45533
タイトル
The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids
著者
Sugihara, Shinji ; Orikasa, Yoshitake ; Okuyama, Hidetoshi
著者名
Sugihara, Shinji
著者名
Orikasa, Yoshitake
著者名
Okuyama, Hidetoshi
言語
英語
キーワード
ASKA clone
EntD
eicosapentaenoic acid
pfaE
phosphopantetheinyl transferase
polyunsaturated fatty acid
発行日
2010-06
出版者
Blackwell Publishing
収録物名
FEMS Microbiology Letters
巻(号)
307(2)
ページ
207-211
抄録
The Escherichia coli entD gene, which encodes an Sfp-type phosphopantetheinyl transferase (PPTase) that is involved in the biosynthesis of siderophore, is available as a high-expression ASKA clone (pCA24N::entD) constructed from the E. coli K-12 strain AG1. In E. coli DH5α, pCA24N::entD complemented a pfaE-deficient clone that comprised pfaA, pfaB, pfaC and pfaD, which are four of the five pfa genes that are responsible for the biosynthesis of eicosapentaenoic acid derived from Shewanella pneumatophori SCRC-2738. Sfp-type PPTases are classified into the EntD and PfaE groups, based on differences between their N-terminal-domain structures. Here, we showed that all Sfp-type PPTases may have the potential to promote the biosynthesis of long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs).
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
The definitive version is available at www.blackwell-synergy.com
PISSN
0378-1097
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1111/j.1574-6968.2010.01987.x