Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes

Matsumoto, Ken'ichiro; Morimoto, Kenjiro; Gohda, Aoi; Shimada, Hiroaki; Taguchi, Seiichi

Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes

Matsumoto, Ken'ichiro; Morimoto, Kenjiro; Gohda, Aoi et al.

Journal of Bioscience and Bioengineering, 2011, 111(4), 485-488

Permalink : https://hdl.handle.net/2115/45613

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/45613
タイトル	Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes
タイトル	翻訳効率の改善による組換え体タバコにおけるバイオプラスチック生産性の向上
著者	Matsumoto, Ken'ichiro ; Morimoto, Kenjiro ; Gohda, Aoi ; Shimada, Hiroaki ; Taguchi, Seiichi
e-Rad_Researcher 80360642 著者名 Matsumoto, Ken'ichiro
著者名 Morimoto, Kenjiro
著者名 Gohda, Aoi
著者名 Shimada, Hiroaki
e-Rad_Researcher 70216828 著者名 Taguchi, Seiichi
言語	英語
キーワード	Transgenic plant
	Nicotiana tabacum
	PHA synthase
	Real-time PCR
発行日	2011-04
出版者	Society for Biotechnology, Japan
収録物名	Journal of Bioscience and Bioengineering
巻(号)	111(4)
ページ	485-488
抄録	Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2 mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold higher than the control transformants harboring the wild-type phaB gene. The immunodetection revealed an increased production of PhaB in leaves, indicating that the enhanced expression of PhaB was effective to increase P(3HB) production in tobacco. In contrast, codon-optimization of the phaC gene exhibited no apparent effect on P(3HB) production. This result suggests the PhaB-catalyzed reaction to be a rate-determining step of P(3HB) biosynthesis in tobacco leaves.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1389-1723
関連情報	PMID 21185778
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jbiosc.2010.11.020