Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy

Mizutani, Yusuke; Tsuchiya, Masahiro; Hiratsuka, Shinichiro; Kawahara, Koichi; Tokumoto, Hiroshi; Okajima, Takaharu

Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy

Mizutani, Yusuke; Tsuchiya, Masahiro; Hiratsuka, Shinichiro et al.

Japanese Journal of Applied Physics, 2008, 47(7), 6177-6180

Permalink : https://hdl.handle.net/2115/45746

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アクセス権	open access
URI	https://hdl.handle.net/2115/45746
タイトル	Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy
著者	Mizutani, Yusuke ; Tsuchiya, Masahiro ; Hiratsuka, Shinichiro ; Kawahara, Koichi ; Tokumoto, Hiroshi ; Okajima, Takaharu
著者名 Mizutani, Yusuke
著者名 Tsuchiya, Masahiro
著者名 Hiratsuka, Shinichiro
e-Rad_Researcher 20125397 著者名 Kawahara, Koichi
e-Rad_Researcher 40357562 著者名 Tokumoto, Hiroshi
e-Rad_Researcher 70280998 著者名 Okajima, Takaharu
言語	英語
キーワード	Atomic force microscope
	Microarray
	Fibroblast cells
	Young's modulus
	Log-normal distribution
発行日	2008-07
出版者	Japan Society of Applied Physics
収録物名	Japanese Journal of Applied Physics
巻(号)	47(7)
ページ	6177-6180
抄録	The number distribution of the elastic modulus of fibroblast cells was successfully measured during the early stages of adhesion using an atomic force microscope (AFM) combined with a microarray as a substrate, which allowed us to arrange and culture cells so that a large number of cells could be measured in a short time period. We confirmed that the cells deposited in the wells of the microarray could be cultured for at least 12 h without any significant migration. Histograms of the Young's modulus, E, of the cells during the early stages of adhesion produced from force curve measurements of cells (n\cong300) cultured for 3–9 h were well fitted to a log-normal distribution function. With increasing incubation time, the average value of E increased significantly, while the standard deviation of the distribution remained almost constant. The results are discussed in terms of the cytoskeleton inside cells.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0021-4922
EISSN	1347-4065
NCID	AA12295836
関連情報	URI http://jjap.jsap.jp/link?JJAP/47/6177/
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1143/JJAP.47.6177