Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells

Yasui, Hironobu; Ito, Nozomi; Yamamori, Tohru; Nakamura, Hideo; Okano, Jun; Asanuma, Taketoshi; Nakajima, Takayuki; Kuwabara, Mikinori; Inanami, Osamu

Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells

Yasui, Hironobu; Ito, Nozomi; Yamamori, Tohru et al.

Free Radical Research, 2010, 44(6), 645-654

Permalink : https://hdl.handle.net/2115/46751

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アクセス権	open access
URI	https://hdl.handle.net/2115/46751
タイトル	Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells
著者	Yasui, Hironobu ; Ito, Nozomi ; Yamamori, Tohru ; Nakamura, Hideo ; Okano, Jun ; Asanuma, Taketoshi ; Nakajima, Takayuki ; Kuwabara, Mikinori ; Inanami, Osamu
e-Rad_Researcher 10570228 著者名 Yasui, Hironobu
著者名 Ito, Nozomi
e-Rad_Researcher 00512675 著者名 Yamamori, Tohru
e-Rad_Researcher 30207906 著者名 Nakamura, Hideo
著者名 Okano, Jun
著者名 Asanuma, Taketoshi
著者名 Nakajima, Takayuki
著者名 Kuwabara, Mikinori
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
言語	英語
キーワード	Antioxidant
	neurogenesis
	nitric oxide
発行日	2010-06
出版者	Informa Healthcare
収録物名	Free Radical Research
巻(号)	44(6)
ページ	645-654
抄録	It has previously been suggested that the spin trap agent α-phenyl-N-tert-butylnitrone (PBN) induces neurite outgrowth through activation of the Ras-ERK pathway in PC12 cells. However, the chemical properties of PBN contributing to its biological function and the detailed mechanism for the activation of Ras by PBN remain unknown. This study demonstrates that the hydrophobic structure of PBN is related to the activation of Ras, by comparing with hydrophilic analogues of PBN. [14C]-labelled PBN was found to localize in the lipid fraction and activate Ras indirectly. On the other hand, neurite outgrowth by PBN was inhibited by a nitric oxide (NO) scavenger. Moreover, the neurite outgrowth induced by PBN and the NO donor NOR4 was inhibited by the dominant negative Ras or MAPK/ERK inhibitor. Taken together, these results suggest that PBN is incorporated into the plasma membrane and induces neurite outgrowth in PC12 cells by activating the Ras-ERK pathway through NO release
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1071-5762
EISSN	1029-2470
NCID	AA11001805
関連情報	PMID 20370559
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.3109/10715761003692537