open access

https://hdl.handle.net/2115/46761

Isolation and Identification of an Anti-Bolting Compound, Hexadecatrienoic Acid Monoglyceride, Responsible for Inhibition of Bolting and Maintenance of the Leaf Rosette in Radish Plants

英語

Sensitivity to gibberellin

2010-08

Oxford University Press

Plant and Cell Physiology

51(8)

1341-1349

Generally, the bolting (stem elongation from rosette plants) of winter annuals is believed to be induced by an increase in the levels of GA that occurs after a certain period of chilling (vernalization), and a deficiency of GA allows the plant to maintain a rosette style. Lack of direct evidence proving the above assumption in radish plants (Raphanus sativus L.) urged us to assume the presence of an anti-bolting compound; actively maintaining the rosette habit through inhibition of bolting. Anti-bolting activity was detected in an extract of rosette shoots of radish plants by an assay using seedlings cultured in vitro. The causal compound that strongly inhibited bolting was isolated and identified as α-(6Z, 9Z, 12Z)-hexadecatrienoic acid monoglyceride (16:3 monoglyceride). This compound did not inhibit leaf production at the apical meristem, indicating that it merely inhibits growth at the internode. The compound disappeared completely after vernalization, and bolting occurred thereafter. The results suggest that the release from inhibition by 16:3 monoglyceride induces the initiation of bolting. The possible mechanism by which the compound exerts the activity is discussed.

学術雑誌論文

Accepted Manuscript

This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version Plant Cell Physiol (2010) 51(8): 1341-1349 is available online at: http://pcp.oxfordjournals.org/content/51/8/1341

0032-0781