Visualization and motility of primordial germ cells using green fluorescent protein fused to 3'UTR of common carp nanos-related gene

Kawakami, Yutaka; Saito, Taiju; Fujimoto, Takafumi; Goto-Kazeto, Rie; Takahashi, Eisuke; Adachi, Shinji; Arai, Katsutoshi; Yamaha, Etsuro

Visualization and motility of primordial germ cells using green fluorescent protein fused to 3'UTR of common carp nanos-related gene

Kawakami, Yutaka; Saito, Taiju; Fujimoto, Takafumi et al.

Aquaculture, 2011, 317(1-4), 245-250

Permalink : https://hdl.handle.net/2115/46772

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アクセス権	open access
URI	https://hdl.handle.net/2115/46772
タイトル	Visualization and motility of primordial germ cells using green fluorescent protein fused to 3'UTR of common carp nanos-related gene
著者	Kawakami, Yutaka ; Saito, Taiju ; Fujimoto, Takafumi ; Goto-Kazeto, Rie ; Takahashi, Eisuke ; Adachi, Shinji ; Arai, Katsutoshi ; Yamaha, Etsuro
著者名 Kawakami, Yutaka
著者名 Saito, Taiju
著者名 Fujimoto, Takafumi
著者名 Goto-Kazeto, Rie
著者名 Takahashi, Eisuke
e-Rad_Researcher 40231930 著者名 Adachi, Shinji
e-Rad_Researcher 00137902 著者名 Arai, Katsutoshi
e-Rad_Researcher 60191376 著者名 Yamaha, Etsuro
言語	英語
キーワード	nanos
	primordial germ cell
	common carp
	goldfish
	germline chimera
発行日	2011-07
出版者	Elsevier
収録物名	Aquaculture
巻(号)	317(1-4)
ページ	245-250
抄録	Primordial germ cells (PGCs) are the only cells in developing embryos with the potential to transmit genetic information to the next generation. We previously visualized the PGCs of several teleostean embryos by injecting RNA synthesized from constructs encoding green fluorescent protein (GFP) fused to the 3'UTR of the zebrafish (Danio rerio) nanos1 gene (nos1). However, this technique was not always suitable for visualizing PGCs in embryos from all teleost species. In this study, we compared the visualization of PGCs in common carp (Cyprinus carpio) embryos using two artificial constructs containing GFP fused to the 3'UTR of nanos from either common carp or zebrafish. Visualization was better using GFP fused to the 3'UTR of the nanos gene from common carp, compared with that from zebrafish. The visualized PGCs successfully migrated toward the gonadal ridge after transplantation into goldfish host embryos, suggesting that they maintained normal migratory motility. These techniques could be useful for the production of inter-specific germline chimeras using common carp donor PGCs.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0044-8486
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.aquaculture.2011.04.019