Formation of 5-formyl-2'-deoxycytidine from 5-methyl-2'-deoxycytidine in duplex DNA by Fenton-type reactions and gamma-irradiation

Murata-Kamiya, Naoko; Kamiya, Hiroyuki; Karino, Naoko et al.

Nucleic Acids Research, 1999, 27(22), 4385-4390

Permalink : https://hdl.handle.net/2115/46962

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アクセス権
open access
URI
https://hdl.handle.net/2115/46962
タイトル
Formation of 5-formyl-2'-deoxycytidine from 5-methyl-2'-deoxycytidine in duplex DNA by Fenton-type reactions and gamma-irradiation
著者
Murata-Kamiya, Naoko ; Kamiya, Hiroyuki ; Karino, Naoko ; Ueno, Yoshihito ; Kaji, Hiroshi ; Matsuda, Akira ; Kasai, Hiroshi
著者名
Murata-Kamiya, Naoko
著者名
Kamiya, Hiroyuki
著者名
Karino, Naoko
著者名
Ueno, Yoshihito
著者名
Kaji, Hiroshi
著者名
Matsuda, Akira
著者名
Kasai, Hiroshi
言語
英語
発行日
1999-11
出版者
Oxford University Press
収録物名
Nucleic Acids Research
巻(号)
27(22)
ページ
4385-4390
抄録
5-Methyl-2′-deoxycytidine (5-Me-dC) is formed by the enzymatic methylation of dC, primarily in CpG sequences in DNA, and is involved in the regulation of gene expression. In the present study, 5-Me-dC and double-stranded DNA fragments containing 5-Me-dC were either γirradiated or aerobically treated with Fenton-type reagents, Fe(ll)-EDTA, Fe(ll)-nitrilotri-acetic acid, Fe(lll)-EDTA-H2O2-catechol or ascorbic acid-H2O2 under neutral conditions. The formation of 5-formyl-2′-deoxycytidine (5-CHO-dC) was observed upon treatment of both 5-Me-dC and DNA fragments containing 5-Me-dC. The yields of 5-CHO-dC from 5-Me-dC and those of 5-formyl-2′-deoxyuridine from dT were comparable. These results suggest that 5-Me-dC in DNA is as susceptible to oxidation as dT in cells, and raise the possibility that 5-CHO-dC may contribute to the high mutagenic rate observed in CpG sequences in genomic DNA.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
EISSN
1362-4962
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1093/nar/27.22.4385