Cloning and functional characterization of Chondrichthyes, cloudy catshark, Scyliorhinus torazame and whale shark, Rhincodon typus estrogen receptors

Katsu, Yoshinao; Kohno, Satomi; Narita, Haruka; Urushitani, Hiroshi; Yamane, Koudai; Hara, Akihiko; Clauss, Tonya M.; Walsh, Michael T.; Miyagawa, Shinichi; Guillette, Louis J.; Iguchi, Taisen

Cloning and functional characterization of Chondrichthyes, cloudy catshark, Scyliorhinus torazame and whale shark, Rhincodon typus estrogen receptors

Katsu, Yoshinao; Kohno, Satomi; Narita, Haruka et al.

General and Comparative Endocrinology, 2010, 168(3), 496-504

Permalink : https://hdl.handle.net/2115/47112

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アクセス権	open access
URI	https://hdl.handle.net/2115/47112
タイトル	Cloning and functional characterization of Chondrichthyes, cloudy catshark, Scyliorhinus torazame and whale shark, Rhincodon typus estrogen receptors
著者	Katsu, Yoshinao ; Kohno, Satomi ; Narita, Haruka ; Urushitani, Hiroshi ; Yamane, Koudai ; Hara, Akihiko ; Clauss, Tonya M. ; Walsh, Michael T. ; Miyagawa, Shinichi ; Guillette, Louis J. ; Iguchi, Taisen
e-Rad_Researcher 00332180 著者名 Katsu, Yoshinao
著者名 Kohno, Satomi
著者名 Narita, Haruka
著者名 Urushitani, Hiroshi
著者名 Yamane, Koudai
著者名 Hara, Akihiko
著者名 Clauss, Tonya M.
著者名 Walsh, Michael T.
著者名 Miyagawa, Shinichi
著者名 Guillette, Louis J.
著者名 Iguchi, Taisen
言語	英語
キーワード	shark
	estrogen receptor
	transactivation
	evolution
	environmental chemicals
発行日	2010-09-15
出版者	Elsevier
収録物名	General and Comparative Endocrinology
巻(号)	168(3)
ページ	496-504
抄録	Sex-steroid hormones are essential for normal reproductive activity in both sexes in all vertebrates. Estrogens are required for ovarian differentiation during a critical developmental stage and promote the growth and differentiation of the female reproductive system following puberty. Recent studies have shown that environmental estrogens influence the developing reproductive system as well as gametogenesis, especially in males. To understand the molecular mechanisms of estrogen actions and to evaluate estrogen receptor ligand interactions in Elasmobranchii, we cloned a single estrogen receptor (ESR) from two shark species, the cloudy catshark (Scyliorhinus torazame) and whale shark (Rhincodon typus) and used an ERE-luciferase reporter assay system to characterize the interaction of these receptors with steroidal and other environmental estrogens. In the transient transfection ERE-luciferase reporter assay system, both shark ESR proteins displayed estrogen-dependent activation of transcription, and shark ESRs were more sensitive to 17β-estradiol compared with other natural and synthetic estrogens. Further, the environmental chemicals, bisphenol A, nonylphenol, octylphenol and DDT could activate both shark ESRs. The assay system provides a tool for future studies examining the receptor-ligand interactions and estrogen disrupting mechanisms in Elasmobranchii.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0016-6480
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ygcen.2010.06.010