Babesia gibsoni : Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis

Yamasaki, Masahiro; Kobayashi, Yusuke; Nakamura, Kensuke; Sasaki, Noboru; Murakami, Masahiro; Rajapakshage, Bandula Kumara Wickramasekara; Ohta, Hiroshi; Yamato, Osamu; Maede, Yoshimitsu; Takiguchi, Mitsuyoshi

Babesia gibsoni : Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis

Yamasaki, Masahiro; Kobayashi, Yusuke; Nakamura, Kensuke et al.

Experimental Parasitology, 2011, 127(1), 119-126

Permalink : https://hdl.handle.net/2115/47276

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アクセス権	open access
URI	https://hdl.handle.net/2115/47276
タイトル	Babesia gibsoni : Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis
著者	Yamasaki, Masahiro ; Kobayashi, Yusuke ; Nakamura, Kensuke ; Sasaki, Noboru ; Murakami, Masahiro ; Rajapakshage, Bandula Kumara Wickramasekara ; Ohta, Hiroshi ; Yamato, Osamu ; Maede, Yoshimitsu ; Takiguchi, Mitsuyoshi
e-Rad_Researcher 40322846 著者名 Yamasaki, Masahiro
著者名 Kobayashi, Yusuke
著者名 Nakamura, Kensuke
著者名 Sasaki, Noboru
著者名 Murakami, Masahiro
著者名 Rajapakshage, Bandula Kumara Wickramasekara
e-Rad_Researcher 50431333 著者名 Ohta, Hiroshi
著者名 Yamato, Osamu
e-Rad_Researcher 40002084 著者名 Maede, Yoshimitsu
e-Rad_Researcher 70261336 著者名 Takiguchi, Mitsuyoshi
言語	英語
キーワード	Babesia gibsoni
	In situ hybridization
	Heat shock protein 70
	Blood smear
	Formalin-fixed
	Paraffin-embedded tissues
発行日	2011-01
出版者	Elsevier
収録物名	Experimental Parasitology
巻(号)	127(1)
ページ	119-126
抄録	In the present study, we attempted to detect Babesia gibsoni in blood smears and formalin-fixed, paraffin-embedded tissues obtained from B. gibsoni-infected dogs using in situ hybridization. Using a digoxigenin-conjugated deoxyribonucleic acid (DNA) probe, both intraerythrocytic and exoerythrocytic parasites in the culture could be specifically stained in blood smears fixed with 4% phosphate-buffered paraformaldehyde. This indicated that genomic DNA extracted from the parasites could be detected using in situ hybridization. Moreover, the parasite could be specifically stained in paraffin-embedded spleen, lymph node, and kidney sections using in situ hybridization. Infected erythrocytes in blood vessels in the spleen and kidney, hemosiderin-laden macrophages in the spleen, and phagocytized erythrocytes, which seemed to be infected with the parasites, in lymph nodes were also specifically stained. This suggests that in situ hybridization can be utilized to investigate both the life cycle of B. gibsoni and the pathological condition of canine babesiosis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-4894
関連情報	PMID 20637756
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.exppara.2010.07.004