Differential Expression of Ezrin and CLP36 in the Two Layers of Syncytiotrophoblast in Rats

Higuchi, Kei; Iizasa, Hisashi; Sai, Yoshimichi; Horieya, Satomi; Lee, Kyeong-Eun; Wada, Masami; Deguchi, Masayuki; Nishimura, Tomohiro; Wakayama, Tomohiko; Tamura, Atsushi; Tsukita, Sachiko; Kose, Noriko; Kang, Young-Sook; Nakashima, Emi

Differential Expression of Ezrin and CLP36 in the Two Layers of Syncytiotrophoblast in Rats

Higuchi, Kei; Iizasa, Hisashi; Sai, Yoshimichi et al.

Biological & Pharmaceutical Bulletin, 2010, 33(8), 1400-1406

Permalink : https://hdl.handle.net/2115/47335

このアイテムのアクセス数:1,389件（2026-03-05 20:12 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
BPB33-8_1400-1406	pdf	8.63 MB	1,146

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/47335
タイトル	Differential Expression of Ezrin and CLP36 in the Two Layers of Syncytiotrophoblast in Rats
著者	Higuchi, Kei ; Iizasa, Hisashi ; Sai, Yoshimichi ; Horieya, Satomi ; Lee, Kyeong-Eun ; Wada, Masami ; Deguchi, Masayuki ; Nishimura, Tomohiro ; Wakayama, Tomohiko ; Tamura, Atsushi ; Tsukita, Sachiko ; Kose, Noriko ; Kang, Young-Sook ; Nakashima, Emi
著者名 Higuchi, Kei
e-Rad_Researcher 80306662 著者名 Iizasa, Hisashi
著者名 Sai, Yoshimichi
著者名 Horieya, Satomi
著者名 Lee, Kyeong-Eun
著者名 Wada, Masami
著者名 Deguchi, Masayuki
著者名 Nishimura, Tomohiro
著者名 Wakayama, Tomohiko
著者名 Tamura, Atsushi
著者名 Tsukita, Sachiko
著者名 Kose, Noriko
著者名 Kang, Young-Sook
著者名 Nakashima, Emi
言語	英語
キーワード	SynI
	SynII
	trophoblast
	ezrin
	blood–placenta barrier
	CLP36
発行日	2010-08
出版者	Pharmaceutical Society of Japan
収録物名	Biological & Pharmaceutical Bulletin
巻(号)	33(8)
ページ	1400-1406
抄録	The syncytiotrophoblast, which regulates maternal-fetal transfer of drugs, consists of a single layer in humans, but two layers, i.e., SynI and SynII, in rodents. Polar distribution of transporters in the apical and basal plasma membranes of syncytiotrophoblast is important for placental function in terms of vectorial transport of substrates, but the mechanisms that control protein distribution in the syncytiotrophoblast remain unclear. We have previously established rat syncytiotrophoblast cell lines, TR-TBT 18d-1 and TR-TBT 18d-2, which retain characteristics of SynI and SynII, respectively. In this study, we aimed to characterize the gene expression profiles in the two layers by using these cell lines. DNA microarray analysis indicated that more than 25 mRNAs, including cytoskeleton binding proteins, ezrin and CLP36, are differentially expressed between TR-TBT 18d-1 and TR-TBT 18d-2. Quantitative real time-polymerase chain reaction (PCR) analysis indicated that mRNA expression of ezrin, CLP36, CCN1, and CCN2 is higher in TR-TBT 18d-1 and mRNA expression of elf-1a, hsc70 and flot2 is higher in TR-TBT 18d-2, compared with their counterparts. Immunohistochemical analysis indicated that ezrin is expressed in rat placental villi in vivo, and is located on the apical membranes of TR-TBT 18d-1, while CLP36 is located in the apical and basal sides of TR-TBT 18d-1. The expression of ezrin was highest at gestational days 14 and 18 and was highest among the ezrin/radixin/moesin (ERM) family members. These results may help to clarify the mechanisms controlling polarization of the syncytiotrophoblast and the significance of the double epithelial layers in rat and mouse.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0918-6158
EISSN	1347-5215
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1248/bpb.33.1400