Design of Mesoporous Silica/Cytosine−Phosphodiester−Guanine Oligodeoxynucleotide Complexes To Enhance Delivery Efficiency

Zhu, Yufang; Meng, Wenjun; Li, Xianglan et al.

The Journal of Physical Chemistry C, 2011, 115(2), 447-452

Permalink : https://hdl.handle.net/2115/47436

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アクセス権
open access
URI
https://hdl.handle.net/2115/47436
タイトル
Design of Mesoporous Silica/Cytosine−Phosphodiester−Guanine Oligodeoxynucleotide Complexes To Enhance Delivery Efficiency
著者
Zhu, Yufang ; Meng, Wenjun ; Li, Xianglan ; Gao, Hong ; Hanagata, Nobutaka
著者名
Zhu, Yufang
著者名
Meng, Wenjun
著者名
Li, Xianglan
著者名
Gao, Hong
著者名
Hanagata, Nobutaka
言語
英語
発行日
2011
出版者
American Chemical Society
収録物名
The Journal of Physical Chemistry C
巻(号)
115(2)
ページ
447-452
抄録
We developed a potential cytosine-phosphodiester-guanine oligodeoxynucleotide (CpG ODN) delivery system combining amino-modified mesoporous silica SBA-15 (SBA-NH2) particles with polycation poly(allylamine hydrochloride) (PAH) to form SBA-ODN-PAH complexes. Stability, cell uptake, in vitro cytotoxicity, and the nuclear factor κB (NF-κB) activity of the SBA-ODN-PAH complexes were evaluated. Gel electrophoresis indicated that the SBA-ODN-PAH complexes exhibited enhanced serum stability due to the dual protection effects of the SBA-NH2 particles and the PAH coating. The SBA-ODN-PAH complexes were taken up by 293XL-hTLR9 cells and little cytotoxicity was expressed in a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Most importantly, the SBA-ODN-PAH complexes significantly enhanced NF-κB activity, stimulated by interaction between the internalized CpG ODN and Toll-like receptor 9, compared to free CpG ODN and SBA-ODN complexes. Thus, SBA-ODN-PAH complexes provide a promising strategy for enhancing the delivery efficiency of CpG ODN.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
1932-7447
EISSN
1932-7455
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1021/jp109535d