Biochemical characterization of the very long-chain fatty acid elongase ELOVL7

Naganuma, Tatsuro; Sato, Yuichiro; Sassa, Takayuki et al.

FEBS Letters, 2011, 585(20), 3337-3341

Permalink : https://hdl.handle.net/2115/47466

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アクセス権	open access
URI	https://hdl.handle.net/2115/47466
タイトル	Biochemical characterization of the very long-chain fatty acid elongase ELOVL7
著者	Naganuma, Tatsuro ; Sato, Yuichiro ; Sassa, Takayuki ; Ohno, Yusuke ; Kihara, Akio
著者名 Naganuma, Tatsuro
著者名 Sato, Yuichiro
著者名 Sassa, Takayuki
著者名 Ohno, Yusuke
e-Rad_Researcher 50333620 著者名 Kihara, Akio
言語	英語
キーワード	Very long-chain fatty acid
	Elongase
	Lipid
	ELOVL
	Membrane
発行日	2011-10-20
出版者	Elsevier B.V.
収録物名	FEBS Letters
巻(号)	585(20)
ページ	3337-3341
抄録	Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we purified ELOVL7 and measured its activity by reconstituting it into proteoliposomes. Purified ELOVL7 exhibited high activity toward acyl-CoAs with C18 carbon chain length. The calculated Km values toward C18: 3-CoA(n-3) and malonyl-CoA were both in the μM range. We also found that progression of the VLCFA cycle enhances ELOVL7 activity.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-5793
関連情報	PMID 21959040
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2011.09.024