open access

https://hdl.handle.net/2115/47497

Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: Comparisons with the normal articular cartilage

英語

2011

BioMed Central

BMC Musculoskeletal Disorders

12(1)

213-224

Background: We have recently found a phenomenon that spontaneous regeneration of a hyaline cartilage-liketissue can be induced in a large osteochondral defect by implanting a double-network (DN) hydrogel plug, whichwas composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N, N’-Dimetyl acrylamide), at thebottom of the defect. The purpose of this study was to clarify gene expression profile of the regenerated tissue incomparison with that of the normal articular cartilage. Methods: We created a cylindrical osteochondral defect in the rabbit femoral grooves. Then, we implanted the DNgel plug at the bottom of the defect. At 2 and 4 weeks after surgery, the regenerated tissue was analyzed usingDNA microarray and immunohistochemical examinations. Results: The gene expression profiles of the regenerated tissues were macroscopically similar to the normalcartilage, but showed some minor differences. The expression degree of COL2A1, COL1A2, COL10A1, DCN, FMOD,SPARC, FLOD2, CHAD, CTGF, and COMP genes was greater in the regenerated tissue than in the normal cartilage.The top 30 genes that expressed 5 times or more in the regenerated tissue as compared with the normal cartilageincluded type-2 collagen, type-10 collagen, FN, vimentin, COMP, EF1alpha, TFCP2, and GAPDH genes. Conclusions: The tissue regenerated by using the DN gel was genetically similar but not completely identical toarticular cartilage. The genetic data shown in this study are useful for future studies to identify specific genesinvolved in spontaneous cartilage regeneration.

学術雑誌論文

Version of Record

Creative Commons Attribution 2.0 Generic

1471-2474

AA12035278