Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction of β-oxidation in Escherichia coli LS5218

Shozui, Fumi; Matsumoto, Ken'ichiro; Motohashi, Ren; Yamada, Miwa; Taguchi, Seiichi

Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction of β-oxidation in Escherichia coli LS5218

Shozui, Fumi; Matsumoto, Ken'ichiro; Motohashi, Ren et al.

Polymer Degradation and Stability, 2010, 95(8), 1340-1344

Permalink : https://hdl.handle.net/2115/47513

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アクセス権	open access
URI	https://hdl.handle.net/2115/47513
タイトル	Establishment of a metabolic pathway to introduce the 3-hydroxyhexanoate unit into LA-based polyesters via a reverse reaction of β-oxidation in Escherichia coli LS5218
著者	Shozui, Fumi ; Matsumoto, Ken'ichiro ; Motohashi, Ren ; Yamada, Miwa ; Taguchi, Seiichi
著者名 Shozui, Fumi
e-Rad_Researcher 80360642 著者名 Matsumoto, Ken'ichiro
著者名 Motohashi, Ren
著者名 Yamada, Miwa
e-Rad_Researcher 70216828 著者名 Taguchi, Seiichi
言語	英語
キーワード	Poly(LA-co-3HB-co-3HHx)
	LA-based polyester
	PhaC1_[Ps](ST/QK)
	Microbial cell factory
	Metabolic engineering
	β-oxidation pathway
発行日	2010-08
出版者	Elsevier
収録物名	Polymer Degradation and Stability
巻(号)	95(8)
ページ	1340-1344
抄録	New lactate (LA)-based terpolymers, P[LA-co-3-hydroxybutyrate (3HB)-co-3-hydroxyhexananoate (3HHx)]s, were produced in recombinant Escherichia coli LS5218 harboring three genes encoding LA-polymerizing enzyme (LPE), propionyl-coenzyme A (CoA) transferase (PCT) and (R)-specific enoyl-CoA hydratase (PhaJ4). When the recombinant LS5218 was grown on glucose with the feeding of butyrate, 3HB-CoA and 3HHx-CoA were supplied, probably via reverse reactions of the β-oxidation pathway and PhaJ4. LPE copolymerized the two monomers with LA-CoA, which was generated by PCT, to yield the terpolymers. Gas chromatography analysis revealed that the terpolymers consisted of 2.7 to 34 mol% LA, 38 to 81 mol% 3HB and 17 to 33 mol% 3HHx units, which can be varied depending on the butyrate concentration fed in the medium. In addition, 1H-13C COSY NMR analysis provided evidence for a linkage between LA and 3HHx units in the polymer.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0141-3910
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.polymdegradstab.2010.01.029