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https://hdl.handle.net/2115/47520

Enhancement of femtosecond laser-induced nucleation of protein in a gel solution

英語

nucleation

2010-01-25

American Institute of Physics

Applied Physics Letters

96(4)

043702

We found that the use of a gel solution with agarose enhanced femtosecond laser-induced nucleation and produced hen egg white lysozyme crystals at three to five times lower supersaturation than those by the femtosecond laser or agarose alone. The fast fluorescence imaging of the protein in the gel solution revealed that cavitation bubbles created high-concentration regions at the focal point, which could be the trigger for protein nucleation. The lower diffusions of protein molecules in agarose gel retained the high-concentration regions for a longer time, and facilitated the nucleation.

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Copyright 2010 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 96, 043702 (2010) and may be found at https://dx.doi.org/10.1063/1.3294622

0003-6951