open access

https://hdl.handle.net/2115/47973

Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans

英語

Streptococcus mutans

2011-12

International Union of Crystallography

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

67(12)

1542-1544

Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2_[1], with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å^[3] Da^[-1]; assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å 3 Da^[-1].

学術雑誌論文

Version of Record

1744-3091