Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy.

Yamada, Yuma; Shinohara, Yasuo; Kakudo, Tomoyuki; Chaki, Shinji; Futaki, Shiroh; Kamiya, Hiroyuki; Harashima, Hideyoshi

Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy.

Yamada, Yuma; Shinohara, Yasuo; Kakudo, Tomoyuki et al.

International journal of pharmaceutics, 2005, 303(1-2), 1-7

Permalink : https://hdl.handle.net/2115/48155

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アクセス権	open access
URI	https://hdl.handle.net/2115/48155
タイトル	Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy.
著者	Yamada, Yuma ; Shinohara, Yasuo ; Kakudo, Tomoyuki ; Chaki, Shinji ; Futaki, Shiroh ; Kamiya, Hiroyuki ; Harashima, Hideyoshi
著者名 Yamada, Yuma
著者名 Shinohara, Yasuo
著者名 Kakudo, Tomoyuki
著者名 Chaki, Shinji
著者名 Futaki, Shiroh
著者名 Kamiya, Hiroyuki
著者名 Harashima, Hideyoshi
言語	英語
キーワード	mastoparan
	transferrrin liposomes
	GALA
	mitochondrial delivery
	anticancer therapy
	apoptosis
発行日	2005-10-13
出版者	Elsevier
収録物名	International journal of pharmaceutics
巻(号)	303(1-2)
ページ	1-7
抄録	Mastoparan (MP), a potent facilitator of mitochondrial permeability transition (PT), could be used as an antitumor agent, if it were encapsulated in a tumor selective delivery system. We recently developed transferrin-modified liposomes (Tf-L) with a pH-sensitive fusogenic peptide (GALA), which delivers an encapsulated fluorescent marker into cytosol efficiently. In this study, we encapsulated MP into Tf-L with GALA for the selective delivery to mitochondria of tumor cells. The MP showed potent PT activity at concentrations above 25 microM in a homogenate of K 562 cells as well as in isolated mitochondria in the presence of phosphate. Tf-L equipped with cholesteryl GALA can release encapsulated sulforhodamine B, while Tf-L failed, as evidenced by confocal laser scanning microscopy. The MP, which was delivered with Tf-L with GALA, released cytochrome c (cyt c) from mitochondria to the cytosol, while free MP released cyt c not only to the cytosol but also extracellulary. These results demonstrate the utility of MP in Tf-L with GALA for cancer therapy.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0378-5173
関連情報	PMID 16139452
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ijpharm.2005.06.009