In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities

Kwon, Hyuck Joon; Yasuda, Kazunori; Ohmiya, Yoshihiro; Honma, Ken-ichi; Chen, Yong Mei; Gong, Jian Ping

In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities

Kwon, Hyuck Joon; Yasuda, Kazunori; Ohmiya, Yoshihiro et al.

Acta Biomaterialia, 2010, 6(2), 494-501

Permalink : https://hdl.handle.net/2115/48157

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アクセス権	open access
URI	https://hdl.handle.net/2115/48157
タイトル	In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities
著者	Kwon, Hyuck Joon ; Yasuda, Kazunori ; Ohmiya, Yoshihiro ; Honma, Ken-ichi ; Chen, Yong Mei ; Gong, Jian Ping
著者名 Kwon, Hyuck Joon
e-Rad_Researcher 20166507 著者名 Yasuda, Kazunori
著者名 Ohmiya, Yoshihiro
著者名 Honma, Ken-ichi
著者名 Chen, Yong Mei
e-Rad_Researcher 20250417 著者名 Gong, Jian Ping
言語	英語
キーワード	ATDC5
	Chondrogenic differentiation
	Poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS)
	Poly(dimethylacrylamide) (PDMAAm)
	Copolymer gels of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and dimethylacrylamide (DMAAm) (P(AMPS-co-DMAAm) gels)
	Type II collagen
	Aggrecan
発行日	2010-02
出版者	Elsevier
収録物名	Acta Biomaterialia
巻(号)	6(2)
ページ	494-501
抄録	We investigated the behavior of chondrogenic ATDC5 cells on synthetic polymer gels with various charge densities: negative charged poly(2-acrylamido-2-methyl-1-propanesulfonic acid) gel, neutral poly(dimethylacrylamide) gel, and copolymer gels of 2-acrylamido-2-methyl-1-propanesulfonic acid and dimethylacrylamide with different compositions (molar fractions of 2-acrylamido-2-methyl-1-propanesulfonic acid, F = 0.25, 0.5, 0.75). In insulin-free maintenance medium, the ATDC5 cells cultured on the highly negatively charged gels; poly (2-acrylamido-2-methyl-1-propanesulfonic acid) gel and the copolymer gels of 2-acrylamido-2-methyl-1-propanesulfonic acid and dimethylacrylamide (F = 0.75), spread and became confluent at day 7, and interestingly formed nodules at day 14, expressing type II collagen and proteoglycan. This result demonstrates that the highly negative charged gels can induce chondrogenic differentiation of ATDC5 cells even in insulin-free maintenance medium, in which the ATDC5 cells cultured on the standard polystyrene dish cannot differentiate into chondrocytes. In insulin-supplemented differentiation medium, ATDC5 cells cultured on the poly (dimethylacrylamide) gel made focal adhesions, rapidly aggregated and formed large nodules within 7 days, expressing significantly greater levels of type II collagen and proteoglycan than cells cultured on the polystyrene dish and the negative charged gels. These results showed that the neutral gel accelerated chondrogenic differentiation of ATDC5 cells cultured in the differentiation medium. We suggest that the highly negatively charged poly (2-acrylamido-2-methyl-1-propanesulfonic acid) gel and the neutral poly (dimethylacrylamide) gel are interesting biomaterials for cartilage tissue engineering as a scaffold with the potential to induce chondrogenic differentiation.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1742-7061
関連情報	PMID 19651251
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.actbio.2009.07.033