Negative-feedback regulation of ATP release : ATP release from cardiomyocytes is strictly regulated during ischemia

Kunugi, Satohiko; Iwabuchi, Sadahiro; Matsuyama, Daisuke; Okajima, Takaharu; Kawahara, Koichi

Negative-feedback regulation of ATP release : ATP release from cardiomyocytes is strictly regulated during ischemia

Kunugi, Satohiko; Iwabuchi, Sadahiro; Matsuyama, Daisuke et al.

Biochemical and Biophysical Research Communications, 2011, 416(3-4), 409-415

Permalink : https://hdl.handle.net/2115/48229

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/48229
タイトル	Negative-feedback regulation of ATP release : ATP release from cardiomyocytes is strictly regulated during ischemia
著者	Kunugi, Satohiko ; Iwabuchi, Sadahiro ; Matsuyama, Daisuke ; Okajima, Takaharu ; Kawahara, Koichi
著者名 Kunugi, Satohiko
著者名 Iwabuchi, Sadahiro
著者名 Matsuyama, Daisuke
e-Rad_Researcher 70280998 著者名 Okajima, Takaharu
e-Rad_Researcher 20125397 著者名 Kawahara, Koichi
言語	英語
キーワード	ATP release
	Cardiomyocytes
	Maxi-anion channel
	Hemichannel
	Purinoceptor
発行日	2011-12-16
出版者	Elsevier
収録物名	Biochemical and Biophysical Research Communications
巻(号)	416(3-4)
ページ	409-415
抄録	Extracellular ATP acts as a potent agonist on cardiomyocytes, inducing a broad range of physiological responses via P2 purinoceptors. Its concentration in the interstitial space within the heart is elevated during ischemia or hypoxia due to its release from a number of cell types, including cardiomyocytes. However, the exact mechanism responsible for the release of ATP from cardiomyocytes during ischemia is not known. In this study, we investigated whether and how the release of ATP was strictly regulated during ischemia in cultured neonatal rat cardiomyocytes. lschemia was mimicked by oxygen-glucose deprivation (OGD). Exposure of cardiomyocytes to OGD resulted in an increase in the concentration of extracellular ATP shortly after the onset of OGD (15 min), and the increase was reversed by treatment with blockers of maxi-anion channels. Unexpectedly, at 1 and 2 hours after the onset of OGD, the blocking of maxi-anion channels increased the concentration of extracellular ATP, and the increase was significantly suppressed by co-treatment with blockers of hemichannels, suggesting that ATP release via maxi-anion channels was involved in the suppression of ATP release via hemichannels during persistent OGD. Here we show the possibility that the release of ATP from cardiomyocytes was strictly regulated during ischemia by negative-feedback mechanisms; that is, maxi-anion channel-derived ATP-induced suppression of ATP release via hemichannels in cardiomyocytes.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 22133679
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2011.11.068