open access

https://hdl.handle.net/2115/48293

Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy

英語

cellular biophysics

2011-12-26

American Institute of Physics

Applied Physics Letters

99(26)

263703

We investigated dynamic force propagation between focal adhesions of fibroblast cells cultured on polydimethylsiloxane micropost substrates, by atomic force microscopy. Live cells were mechanically modulated by the atomic force microscopy probe bound to cell apical surfaces at 0.01-0.5 Hz, while microposts served as a force sensor at basal surfaces. We observed that cells exhibited rheological behavior at the apical surface but had no apparent out-of-phase response at the basal surface, indicating that the dynamic force propagating through cytoskeletal filaments behaves in an elastic manner. Moreover, the direction of the propagated force was observed to be intimately associated with the prestress.

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Copyright 2011 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 99, 263703 (2011) and may be found at https://dx.doi.org/10.1063/1.3672225

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