Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)

Nagasato, Chikako; Inoue, Akira; Mizuno, Masashi; Kanazawa, Kazuki; Ojima, Takao; Okuda, Kazuo; Motomura, Taizo

Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)

Nagasato, Chikako; Inoue, Akira; Mizuno, Masashi et al.

Planta, 2010, 232(2), 287-298

Permalink : https://hdl.handle.net/2115/48362

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アクセス権	open access
URI	https://hdl.handle.net/2115/48362
タイトル	Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)
著者	Nagasato, Chikako ; Inoue, Akira ; Mizuno, Masashi ; Kanazawa, Kazuki ; Ojima, Takao ; Okuda, Kazuo ; Motomura, Taizo
e-Rad_Researcher 00374710 著者名 Nagasato, Chikako
e-Rad_Researcher 70396307 著者名 Inoue, Akira
著者名 Mizuno, Masashi
著者名 Kanazawa, Kazuki
e-Rad_Researcher 30160865 著者名 Ojima, Takao
著者名 Okuda, Kazuo
e-Rad_Researcher 30183974 著者名 Motomura, Taizo
言語	英語
キーワード	brown algae
	cell wall
	cytokinesis
	flat cisternae
	Golgi derived vesicles
発行日	2010-07
出版者	Springer Berlin / Heidelberg
収録物名	Planta
巻(号)	232(2)
ページ	287-298
抄録	During cytokinesis in brown algal cells, Golgi derived vesicles (GVs) and flat cisternae (FCs) are involved in building the new cell partition membrane. In this study, we followed the membrane fusion process in Silvetia babingtonii zygotes using electron microscopy together with rapid freezing and freeze substitution. After mitosis, many FCs were formed around endoplasmic reticulum clusters and these then spread toward the future cytokinetic plane. Actin depolymerisation using latrunculin B prevented the appearance of the FCs. Fusion of GVs to FCs resulted in structures that were thicker and more elongated (EFCs; expanded flat cisternae). Some complicated membranous structures (MN; membranous network) were formed by interconnection of EFCs and following the arrival of additional GVs. The MN grew into membranous sacs (MSs) as gaps between the MNs disappeared. The MSs were observed in patches along the cytokinetic plane. Neighboring MSs were united to form the new cell partition membrane. An immunocytochemical analysis indicated that fucoidan was synthesized in Golgi bodies and transported by vesicles to the future cytokinetic plane, where the vesicles fused with the FCs. Alginate was not detected until the MS phase. Incubation of sections with cellulase-gold showed that the cellulose content of the new cross wall was not comparable to that of the parent cell wall.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0032-0935
EISSN	1432-2048
関連情報	PMID 20473516
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00425-010-1188-8