Structural properties of trypsin from cold-adapted fish, arabesque greenling (Pleurogrammus azonus)

Kanno, Gaku; Kishimura, Hideki; Ando, Seiichi; Klomklao, Sappasith; Nalinanon, Sitthipong; Benjakul, Soottawat; Chun, Byung-Soo; Saeki, Hiroki

Structural properties of trypsin from cold-adapted fish, arabesque greenling (Pleurogrammus azonus)

Kanno, Gaku; Kishimura, Hideki; Ando, Seiichi et al.

European Food Research and Technology, 2011, 232(3), 381-388

Permalink : https://hdl.handle.net/2115/48491

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アクセス権	open access
URI	https://hdl.handle.net/2115/48491
タイトル	Structural properties of trypsin from cold-adapted fish, arabesque greenling (Pleurogrammus azonus)
著者	Kanno, Gaku ; Kishimura, Hideki ; Ando, Seiichi ; Klomklao, Sappasith ; Nalinanon, Sitthipong ; Benjakul, Soottawat ; Chun, Byung-Soo ; Saeki, Hiroki
著者名 Kanno, Gaku
e-Rad_Researcher 50204855 著者名 Kishimura, Hideki
著者名 Ando, Seiichi
著者名 Klomklao, Sappasith
著者名 Nalinanon, Sitthipong
著者名 Benjakul, Soottawat
著者名 Chun, Byung-Soo
e-Rad_Researcher 90250505 著者名 Saeki, Hiroki
言語	英語
キーワード	Trypsin
	Pyloric caecum
	Arabesque greenling
	Pleurogrammus azonus
	Primary structure
	Thermostability
	Cold-adaptation
発行日	2011-03
出版者	Springer Berlin / Heidelberg
収録物名	European Food Research and Technology
巻(号)	232(3)
ページ	381-388
抄録	A cDNA clone encoding trypsin (AG-T) was isolated from the pyloric ceca of cold-adapted fish, arabesque greenling (Pleurogrammus azonus). The cDNA was composed of 892 bp with an open reading frame of 729 bp at nucleotide positions 25-753. Similar to all the known trypsin, the AG-T seemed to be synthesized as preproenzyme that contains a hydrophobic signal peptide, an activation pentapeptide and a mature trypsin of 222 amino acid residues. The AG-T also completely conserved the major structural features common to trypsin such as the catalytic triad (His57, Asp102, and Ser195), the obligatory Asp189 and twelve Cys residues. On the other hand, the AG-T possessed the deletion of Tyr151 and substitution of Pro152 for Gly in the autolysis loop when aligned with the sequence of tropical-zone fish and bovine trypsins. In addition, Val75 concerned in a combination with calcium ion was exchanged for Ala in the AG-T, and the content of positively charged amino acid residues at the calcium-binding site of the AG-T was three times higher than those of tropical-zone fish trypsins. Moreover, the ratio between charged and hydrophobic amino acid residues in the N-terminal region of the AG-T was also higher than those of temperate-zone fish and tropical-zone fish trypsins. Such structural properties of the AG-T would contribute to its low thermostability.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	1438-2377
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00217-010-1404-6