Ciliates promote the transfer of the gene encoding the extended-spectrum  -lactamase CTX-M-27 between Escherichia coli strains

Oguri, Satoshi; Matsuo, Junji; Hayashi, Yasuhiro; Nakamura, Shinji; Hanawa, Tomoko; Fukumoto, Tatsuya; Mizutani, Yoshihiko; Yao, Takashi; Akizawa, Kouzi; Suzuki, Haruki; Shimizu, Chikara; Matsuno, Kazuhiko; Kamiya, Shigeru; Yamaguchi, Hiroyuki

Ciliates promote the transfer of the gene encoding the extended-spectrum -lactamase CTX-M-27 between Escherichia coli strains

Oguri, Satoshi; Matsuo, Junji; Hayashi, Yasuhiro et al.

Journal of Antimicrobial Chemotherapy, 2011, 66(3), 527-530

Permalink : https://hdl.handle.net/2115/48508

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アクセス権	open access
URI	https://hdl.handle.net/2115/48508
タイトル	Ciliates promote the transfer of the gene encoding the extended-spectrum -lactamase CTX-M-27 between Escherichia coli strains
著者	Oguri, Satoshi ; Matsuo, Junji ; Hayashi, Yasuhiro ; Nakamura, Shinji ; Hanawa, Tomoko ; Fukumoto, Tatsuya ; Mizutani, Yoshihiko ; Yao, Takashi ; Akizawa, Kouzi ; Suzuki, Haruki ; Shimizu, Chikara ; Matsuno, Kazuhiko ; Kamiya, Shigeru ; Yamaguchi, Hiroyuki
著者名 Oguri, Satoshi
e-Rad_Researcher 50359486 著者名 Matsuo, Junji
e-Rad_Researcher 30548178 著者名 Hayashi, Yasuhiro
e-Rad_Researcher 40207882 著者名 Nakamura, Shinji
著者名 Hanawa, Tomoko
著者名 Fukumoto, Tatsuya
著者名 Mizutani, Yoshihiko
著者名 Yao, Takashi
著者名 Akizawa, Kouzi
著者名 Suzuki, Haruki
e-Rad_Researcher 00292029 著者名 Shimizu, Chikara
e-Rad_Researcher 70102332 著者名 Matsuno, Kazuhiko
著者名 Kamiya, Shigeru
e-Rad_Researcher 40221650 著者名 Yamaguchi, Hiroyuki
言語	英語
キーワード	ciliates
	gene transfer
	Escherichia coli
	ESBL
	CTX-M-27
発行日	2011-03
出版者	The British Society for Antimicrobial Chemotherapy
収録物名	Journal of Antimicrobial Chemotherapy
巻(号)	66(3)
ページ	527-530
抄録	OBJECTIVES:The mechanism by which Escherichia coli acquires multidrug resistance genes from other bacteria in the natural environment or livestock is still unclear. The ability of ciliates to promote the transfer of genes encoding extended-spectrum β-lactamases (ESBLs) between the CTX-M-27 donor and clinically isolated recipient E. coli strains was investigated. METHODS:Equal amounts (∼10(9) cfu) of donor cefotaxime-resistant E. coli and recipient ciprofloxacin-resistant E. coli strains were mixed together in the presence or absence of 10(5) ciliates in Page's amoeba saline for 24 h, in the presence or absence of certain drugs (cytochalasin D, cycloheximide and latrunculin B). RESULTS:Gene transfer frequency in the presence of ciliates was estimated at ∼10(-6); in the absence of ciliates it was ∼10(-10). Protein synthesis (cycloheximide) or phagocytosis (cytochalasin D or latrunculin B) inhibitors significantly reduced the frequency of gene transfer. CONCLUSIONS:Ciliates promote the transfer of genes encoding ESBLs between E. coli strains, implying that the presence of ciliates may provide a significant impact on emerging multidrug-resistant bacteria.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version is available online at: http://jac.oxfordjournals.org/content/66/3/527.full
PISSN	0305-7453
EISSN	1460-2091
関連情報	PMID 21177671
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/jac/dkq487