Development of multiple-locus variable-number tandem-repeat analysis for rapid genotyping of Ehrlichia ruminantium and its application to infected Amblyomma variegatum collected in heartwater endemic areas in Uganda

Nakao, Ryo; Morrison, Liam J.; Zhou, Lijia; Magona, Joseph W.; Jongejan, Frans; Sugimoto, Chihiro

Development of multiple-locus variable-number tandem-repeat analysis for rapid genotyping of Ehrlichia ruminantium and its application to infected Amblyomma variegatum collected in heartwater endemic areas in Uganda

Nakao, Ryo; Morrison, Liam J.; Zhou, Lijia et al.

Parasitology, 2012, 139(1), 69-82

Permalink : https://hdl.handle.net/2115/48521

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アクセス権	open access
URI	https://hdl.handle.net/2115/48521
タイトル	Development of multiple-locus variable-number tandem-repeat analysis for rapid genotyping of Ehrlichia ruminantium and its application to infected Amblyomma variegatum collected in heartwater endemic areas in Uganda
著者	Nakao, Ryo ; Morrison, Liam J. ; Zhou, Lijia ; Magona, Joseph W. ; Jongejan, Frans ; Sugimoto, Chihiro
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
著者名 Morrison, Liam J.
著者名 Zhou, Lijia
著者名 Magona, Joseph W.
著者名 Jongejan, Frans
e-Rad_Researcher 90231373 著者名 Sugimoto, Chihiro
言語	英語
キーワード	Ehrlichia ruminantium
	heartwater
	Amblyomma variegation
	MLVA
	VNTR
	ticks
発行日	2012-01
出版者	Cambridge University Press
収録物名	Parasitology
巻(号)	139(1)
ページ	69-82
抄録	The rickettsial bacterium Ehrlichia ruminantium is the causative agent of heartwater, a serious tick-borne disease in ruminants. The genetic diversity of organisms in the field will have implications for cross-protective capacities of any vaccine developed, and for an effective vaccine design strategy proper genotyping and understanding of existing genetic diversity in the field is necessary. We searched for variable-number tandem-repeat (VNTR) loci for use in a multi-locus VNTR analysis (MLVA). Sequencing analysis of 30 potential VNTRs using a panel of 16 reference strains from geographically diverse origins identified 12 VNTRs with allelic profiles differing between strains. Application of MLVA to 38 E. ruminantium-infected Amblyomma variegatum collected from indigenous cattle in six different districts of Uganda identified 21 MLVA types. The discriminatory power of MLVA was greater than that of map1 PCR-restriction fragment length polymorphism analysis, with which only six genotypes were obtained. The high discriminatory power as well as cost effective performance of MLVA provide the potential for this technique to be applied in the future with respect to optimizing vaccine trials by identifying local strain diversity, and also raise the possibility of exploring the association between E. ruminantium genotypes and phenotypes such as pathological outcome in the ruminant host.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Copyright © Cambridge University Press 2011
PISSN	0031-1820
関連情報	PMID 22008706
関連情報	URI http://journals.cambridge.org/action/displayJournal?jid=PAR
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1017/S003118201100165X