Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Fujita, Megumi; Kato, Hirotomo; Cáceres, Abraham G.; Gomez, Eduardo A.; Velez, Lenin; Mimori, Tatsuyuki; Zhang, Feifei; Iwata, Hiroyuki; Korenaga, Masataka; Sakurai, Tatsuya; Katakura, Ken; Hashiguchi, Yoshihisa

Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Fujita, Megumi; Kato, Hirotomo; Cáceres, Abraham G. et al.

Acta Tropica, 2012, 121(2), 93-98

Permalink : https://hdl.handle.net/2115/48545

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アクセス権	open access
URI	https://hdl.handle.net/2115/48545
タイトル	Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic
著者	Fujita, Megumi ; Kato, Hirotomo ; Cáceres, Abraham G. ; Gomez, Eduardo A. ; Velez, Lenin ; Mimori, Tatsuyuki ; Zhang, Feifei ; Iwata, Hiroyuki ; Korenaga, Masataka ; Sakurai, Tatsuya ; Katakura, Ken ; Hashiguchi, Yoshihisa
著者名 Fujita, Megumi
e-Rad_Researcher 00346579 著者名 Kato, Hirotomo
著者名 Cáceres, Abraham G.
著者名 Gomez, Eduardo A.
著者名 Velez, Lenin
e-Rad_Researcher 00117384 著者名 Mimori, Tatsuyuki
著者名 Zhang, Feifei
e-Rad_Researcher 40193750 著者名 Iwata, Hiroyuki
著者名 Korenaga, Masataka
著者名 Sakurai, Tatsuya
e-Rad_Researcher 10130155 著者名 Katakura, Ken
e-Rad_Researcher 10037385 著者名 Hashiguchi, Yoshihisa
言語	英語
キーワード	Genotyping
	Sand fly
	Lutzomyia
	PCR-RFLP
	18S rRNA gene
発行日	2012-02
出版者	Elsevier B.V.
収録物名	Acta Tropica
巻(号)	121(2)
ページ	93-98
抄録	Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0001-706X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.actatropica.2011.10.004