White clover mosaic virus-induced gene silencing in pea

Ido, Yukari; Nakahara, Kenji S; Uyeda, Ichiro

Journal of General Plant Pathology, 2012, 78(2), 127-132

Permalink : https://hdl.handle.net/2115/48569

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アクセス権	open access
URI	https://hdl.handle.net/2115/48569
タイトル	White clover mosaic virus-induced gene silencing in pea
著者	Ido, Yukari ; Nakahara, Kenji S ; Uyeda, Ichiro
著者名 Ido, Yukari
e-Rad_Researcher 90315606 著者名 Nakahara, Kenji S
e-Rad_Researcher 10113523 著者名 Uyeda, Ichiro
言語	英語
キーワード	Pea
	RNA silencing
	Potexvirus
発行日	2012
出版者	Springer
収録物名	Journal of General Plant Pathology
巻(号)	78(2)
ページ	127-132
抄録	Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS). VIGS is a powerful tool for modifying gene expression in host plants. We constructed a virus vector based on White clover mosaic virus (WClMV) and demonstrated VIGS of phytoene desaturase (PDS) in pea. Photobleaching of tissues, caused by VIGS of PDS, was observed in restricted areas of upper leaves and stems. We confirmed that the PDS mRNA and subgenomic RNAs of WClMV were reduced in the photobleached tissues.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1345-2630
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10327-012-0360-3