Mechanism for propofol inhibition of Na＋, K＋-ATPase activity in rat brain

Hase, Yuri; Deyama, Yoshiaki; Yoshimura, Yoshitaka; Suzuki, Kuniaki; Fukushima, Kazuaki

Mechanism for propofol inhibition of Na＋, K＋-ATPase activity in rat brain

Hase, Yuri; Deyama, Yoshiaki; Yoshimura, Yoshitaka et al.

北海道歯学雑誌, 2012, 32(2), 147-155

Permalink : https://hdl.handle.net/2115/48709

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アクセス権	open access
URI	https://hdl.handle.net/2115/48709
タイトル	Mechanism for propofol inhibition of Na＋, K＋-ATPase activity in rat brain
著者	Hase, Yuri ; Deyama, Yoshiaki ; Yoshimura, Yoshitaka ; Suzuki, Kuniaki ; Fukushima, Kazuaki
著者名 Hase, Yuri
e-Rad_Researcher 80271667 著者名 Deyama, Yoshiaki
e-Rad_Researcher 30230816 著者名 Yoshimura, Yoshitaka
e-Rad_Researcher 40133748 著者名 Suzuki, Kuniaki
e-Rad_Researcher 00002361 著者名 Fukushima, Kazuaki
言語	英語
キーワード	Intravenous anesthetic
	propofol
	Na＋
	K＋-ATPase
発行日	2012-03
出版者	北海道歯学会
出版者	Hokkaido association for dental science
収録物名	北海道歯学雑誌
収録物名	Hokkaido journal of dental science
巻(号)	32(2)
ページ	147-155
抄録	Propofol is one of the most widely used intravenous anesthetics, however the mechanism of the anesthetic effect is not fully understood. Na+,K+-ATPase is an enzyme present in all animal cell membranes and plays essential roles for the maintenance of neuronal excitability. There is a report of propofol inhibition of Na+,K+-ATPase activity, but the mechanism is not clearly established. To study the mechanism for propofol inhibition of Na+,K+-ATPase purified from whole brains of rats, the effects of propofol on Na+,K+-ATPase activity, Na+-ATPase, and K+-pNPPase activities, which are partial reactions of Na+,K+-ATPase were examined. Na+,K+-ATPase and Na+-ATPase activities decreased depending on the concentration of propofol, and were completely inhibited at 1.03 mM. Propofol decreased the maximum activity of Na+-, K+-, Mg2+-, and ATPdependent activation of Na+,K+-ATPase activity depending on its concentration, and changed the half maximal concentration for Na+, K+, and ATP, but not for Mg2+. Propofol also decreased the maximum activities of Na+-ATPase and K+-pNPPase, suggesting that propofol inhibits Na+,K+-ATPase activity by affecting the whole reaction process of Na+,K+-ATPase. The inhibition of Na+,K+-ATPase activity by propofol was reversible by dilution of its concentration. These results suggest that propofol reversibly inhibits Na+,K+-ATPase activity in a mixed-type inhibition pattern.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0914-7063
NCID	AN00229778