open access

https://hdl.handle.net/2115/49037

Crystal structure of the octameric pore of staphylococcal γ-hemolysin reveals the β-barrel pore formation mechanism by two components

英語

2011-10-18

National Academy of Sciences

Proceedings of the National Academy of Sciences of the United States of America

108(42)

17314-17319

Staphylococcal γ-hemolysin is a bi-component pore-forming toxin composed of LukF and Hlg2. These proteins are expressed as water-soluble monomers and then assemble into the oligomeric pore form on the target cell. Here, we report the crystal structure of the octameric pore form of γ-hemolysin at 2.5Å resolution, which is the first high-resolution structure of a β-barrel transmembrane protein composed of two proteins reported to date. The octameric assembly consists of four molecules of LukF and Hlg2 located alternately in a circular pattern, which explains the biochemical data accumulated over the past two decades. The structure, in combination with the monomeric forms, demonstrates the elaborate molecular machinery involved in pore formation by two different molecules, in which inter-protomer electrostatic interactions using loops connecting β2 and β3 (loop A:Asp43-Lys48 of LukF and Lys37-Lys43 of Hlg2) play pivotal roles as the structural determinants for assembly through unwinding of the N-terminal β-strands (amino-latch) of the adjacent protomer, releasing the transmembrane stem domain folded into a β-sheet in the monomer (pre-stem), and interaction with the adjacent protomer.

学術雑誌論文

Accepted Manuscript

0027-8424