open access

https://hdl.handle.net/2115/49095

Cryopreservation tolerance of Marine Diatom Resting Stage Cells in the Coastal Sea Bottom Sediments

日本語

Nitzschia sp.

2012-03-28

北海道大学大学院水産科学研究院

Bulletin of Fisheries Sciences, Hokkaido University

62(1)

15-19

In spite of its importance in microbiology, information on cryopreservation of marine diatom is limited.　An attempt was made to develop cryopreservation procedure of marine diatom resting stage cells which abundantly exist in the bottom sediment of coastal area.　Bottom sediment samples from the Seto Inland Sea (Harima-Nada) were used for sub-zero temperature preservation experiment in this study.　The condition giving optimal survival of microalgae in cryopreserved sediments was as follows : with sorbitol (1 M) as additives, 1/3 salinity, at －80°C preservation (no pre-cooling).　Under this condition, two diatoms, Thalassiosira pseudonana and Nitzschia sp., and some unidentified microalgae were recovered from the sediments, but the vegetative cells of obtained diatoms were not able to be cryopreserved again under the same conditions.　Present result suggests that these two diatoms were preserved as resting stage cells in the coastal bottom sediments, and the diatom resting stage cells might be more resistant to cryopreservation than the vegetative cells.

紀要論文

Version of Record

1346-1842

AA11547977