Phenotypic grouping of 141 BmNPVs lacking viral gene sequences

Ono, Chikako; Kamagata, Takanori; Taka, Hitomi et al.

Virus Research, 2012, 165(2), 197-206

Permalink : https://hdl.handle.net/2115/49265

このアイテムのアクセス数:1,800件（2026-10-11 06:56 集計）

閲覧可能ファイル 

ファイル フォーマット サイズ 閲覧回数 説明
VR165-2_197-206 pdf 616 KB 2,155

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
open access
URI
https://hdl.handle.net/2115/49265
タイトル
Phenotypic grouping of 141 BmNPVs lacking viral gene sequences
著者
Ono, Chikako ; Kamagata, Takanori ; Taka, Hitomi ; Sahara, Ken ; Asano, Shin-ichiro ; Bando, Hisanori
著者名
Ono, Chikako
著者名
Kamagata, Takanori
著者名
Taka, Hitomi
e-Rad_Researcher
30241368
著者名
Sahara, Ken
e-Rad_Researcher
60222585
著者名
Asano, Shin-ichiro
e-Rad_Researcher
20189731
著者名
Bando, Hisanori
言語
英語
キーワード
BmNPV
Bacmid
Lambda red recombination system
Gene knockout
発行日
2012-05
出版者
Elsevier B.V.
収録物名
Virus Research
巻(号)
165(2)
ページ
197-206
抄録
We constructed a series of gene knockout BmNPVs (KOVs) for each of 141 genes using the BmNPV T3 bacmid system and lambda red recombination system. In a subsequent analysis of the properties needed for infection using a marker gene, egfp (enhanced green fluorescent protein gene), inserted into the polyhedrin locus, the knockout viruses (KOVs) were subdivided into four phenotypic types, A to D. Type-A (86 KOVs) showed the ability to expand infections equivalent to the control while type-B (8 KOVs) spread infections more slowly. Type-C (37 KOVs) expressed egfp in transfected-BmN cells but the production of infectious viruses was not observed. Type-D (10 KOVs) showed no ability to express egfp even in the transfection experiments. KOVs lacking genes (pkip (Bm15), gp41 (Bm66), bro-d (Bm131), Bm20, 48, 65, 91, 93, or 101) previously identified as being essential, were placed in the viable type-A and B categories.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0168-1702
関連情報
PMID
22421381
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.virusres.2012.02.016