Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea

Atsumi, Go; Nakahara, Kenji S; Wada, Tomoko Sugikawa; Choi, Sun Hee; Masuta, Chikara; Uyeda, Ichiro

Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea

Atsumi, Go; Nakahara, Kenji S; Wada, Tomoko Sugikawa et al.

Archives of Virology, 2012, 157(6), 1019-1028

Permalink : https://hdl.handle.net/2115/49369

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アクセス権	open access
URI	https://hdl.handle.net/2115/49369
タイトル	Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
著者	Atsumi, Go ; Nakahara, Kenji S ; Wada, Tomoko Sugikawa ; Choi, Sun Hee ; Masuta, Chikara ; Uyeda, Ichiro
著者名 Atsumi, Go
e-Rad_Researcher 90315606 著者名 Nakahara, Kenji S
著者名 Wada, Tomoko Sugikawa
著者名 Choi, Sun Hee
e-Rad_Researcher 60281854 著者名 Masuta, Chikara
e-Rad_Researcher 10113523 著者名 Uyeda, Ichiro
言語	英語
発行日	2012
出版者	Springer
収録物名	Archives of Virology
巻(号)	157(6)
ページ	1019-1028
抄録	Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.
資源タイプ	学術雑誌論文
出版タイプ	Submitted Manuscript Under Review
PISSN	0304-8608
関連情報	PMID 22398917
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00705-012-1281-3