open access

https://hdl.handle.net/2115/49883

Chiral separation of glycerolipids by high-performance liquid chromatography

英語

Mass spectrometry

2012

日本脂質栄養学会

Journal of Lipid Nutrition

21(1)

27-34

Since the enantiomer resolution of synthetic mono- and diacylglycerols as 3,5-dinitrophenylurethanes was achieved by chiral-phase high-performance liquid chromatography (HPLC) in the 1980s, the methodology has been extended to the resolution of various synthetic and naturally occurring chiral glycerolipids. The recent development of polysaccharide-based chiral stationary phases along with optimized chromatographic elution conditions has permitted direct enantiomer resolution of mono-, di-, and triacylglycerols without previous derivatization. However, there are still some compounds for which enantiomer resolution is difficult to achieve, such as phosphatidylcholine and phosphatidylethanolamine. With the development of new derivatization reagents and chromatographic techniques, resolution of these difficult compounds may be possible in the near future. Chiral-phase HPLC will become considerably important in the future as a valuable tool for further expansion of the field of lipidomics and a better understanding of lipid metabolism.

学術雑誌論文

Version of Record

1343-4594

1883-2237