New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes.

Fujimori, Chika; Ogiwara, Katsueki; Hagiwara, Akane; Takahashi, Takayuki

New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes.

Fujimori, Chika; Ogiwara, Katsueki; Hagiwara, Akane et al.

Molecular and cellular endocrinology, 2012, 362(1-2), 76-84

Permalink : https://hdl.handle.net/2115/49982

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アクセス権	open access
URI	https://hdl.handle.net/2115/49982
タイトル	New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes.
著者	Fujimori, Chika ; Ogiwara, Katsueki ; Hagiwara, Akane ; Takahashi, Takayuki
著者名 Fujimori, Chika
e-Rad_Researcher 00422006 著者名 Ogiwara, Katsueki
著者名 Hagiwara, Akane
e-Rad_Researcher 80197152 著者名 Takahashi, Takayuki
言語	英語
キーワード	Prostaglandin E2
	EP receptor
	Intracellular cAMP level
	Gene expression
	Ovulation
	Medaka
発行日	2012-10-15
出版者	Elsevier Science
収録物名	Molecular and cellular endocrinology
巻(号)	362(1-2)
ページ	76-84
抄録	A cDNA for a prostaglandin E(2) (PGE(2)) receptor subtype 4, EP4b (Ptger4b), was cloned from the medaka ovary. The effect of PGE(2) was examined using COS-7 cells expressing the recombinant Ptger4b protein. An increase in intracellular cAMP levels was observed when the cells were incubated with PGE(2), but the increase in cAMP levels was nullified by the addition of the EP4 antagonist GW627368X. The expression of ptger4b mRNA was drastically induced by the addition of pregnant mare serum gonadotropin to the in vitro culture of large preovulatory follicles. In in vitro ovulation studies of the effect of GW627368X addition on follicle ovulation, the critical timing of the PGE(2)/Ptger4b interaction was suggested to be between -1 and 0h of ovulation. These results further substantiate that PGE(2)/Ptger4b signaling is involved in follicle rupture during ovulation in the medaka ovary.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1872-8057
関連情報	PMID 22659410
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.mce.2012.05.013