The Peptide Nucleic Acid-Locked Nucleic Acid Polymerase Chain Reaction Clamp-Based Test for Epidermal Growth Factor Receptor Mutations in Bronchoscopic Cytological Specimens of Non-Small Cell Lung Cancer

Yamada, Noriyuki; Oizumi, Satoshi; Asahina, Hajime; Shinagawa, Naofumi; Kikuchi, Eiki; Kikuchi, Junko; Sakakibara-Konishi, Jun; Tanaka, Tomoaki; Kobayashi, Kunihiko; Hagiwara, Koichi; Nishimura, Masaharu

The Peptide Nucleic Acid-Locked Nucleic Acid Polymerase Chain Reaction Clamp-Based Test for Epidermal Growth Factor Receptor Mutations in Bronchoscopic Cytological Specimens of Non-Small Cell Lung Cancer

Yamada, Noriyuki; Oizumi, Satoshi; Asahina, Hajime et al.

Oncology, 2012, 82(6), 341-346

Permalink : https://hdl.handle.net/2115/50057

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アクセス権	open access
URI	https://hdl.handle.net/2115/50057
タイトル	The Peptide Nucleic Acid-Locked Nucleic Acid Polymerase Chain Reaction Clamp-Based Test for Epidermal Growth Factor Receptor Mutations in Bronchoscopic Cytological Specimens of Non-Small Cell Lung Cancer
著者	Yamada, Noriyuki ; Oizumi, Satoshi ; Asahina, Hajime ; Shinagawa, Naofumi ; Kikuchi, Eiki ; Kikuchi, Junko ; Sakakibara-Konishi, Jun ; Tanaka, Tomoaki ; Kobayashi, Kunihiko ; Hagiwara, Koichi ; Nishimura, Masaharu
著者名 Yamada, Noriyuki
e-Rad_Researcher 10421968 著者名 Oizumi, Satoshi
著者名 Asahina, Hajime
著者名 Shinagawa, Naofumi
e-Rad_Researcher 60463741 著者名 Kikuchi, Eiki
著者名 Kikuchi, Junko
e-Rad_Researcher 50374278 著者名 Sakakibara-Konishi, Jun
著者名 Tanaka, Tomoaki
著者名 Kobayashi, Kunihiko
著者名 Hagiwara, Koichi
e-Rad_Researcher 00208224 著者名 Nishimura, Masaharu
言語	英語
キーワード	Non-small cell lung cancer
	Epidermal growth factor receptor mutations
	Bronchoscopy
	Cytological specimens
	EGFR mutations
発行日	2012-07
出版者	Karger
収録物名	Oncology
巻(号)	82(6)
ページ	341-346
抄録	Objectives: Cytological examination of samples obtained by bronchoscopy is a useful method for establishing the diagnosis of non-small cell lung cancer (NSCLC). However, the utility of a highly sensitive method for the detection of epidermal growth factor receptor (EGFR) mutation in the cytological specimens has not been fully evaluated. Methods: We retrospectively examined the efficacy of the peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp (PNA-LNA PCR clamp) method for detecting EGFR mutations in 122 bronchoscopic cytological specimens from NSCLC patients. Results: Overall, 41 specimens (33.6%) were positive for EGFR mutation. Twenty-nine (39.7%) of 73 specimens obtained by using endobronchial ultrasonography with a guide sheath, 7 (33.3%) of 21 specimens obtained under direct vision by using a conventional bronchoscope, 4 (36.4%) of 11 specimens obtained by using an ultrathin bronchoscope, and 1 (5.9%) of 17 specimens obtained by endobronchial ultrasound-guided transbronchial needle aspiration were positive for EGFR mutation. Furthermore, among 22 resected NSCLC cases, the EGFR mutation status obtained from bronchoscopic materials was consistent with the status obtained from surgical samples, with the exception of one case. Conclusion: The detection of EGFR mutation by subjecting bronchoscopic cytological specimens to a PNA-LNA PCR clamp assay proves useful.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	© 2012 S. Karger AG, Basel
PISSN	0030-2414
関連情報	PMID 22677909
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1159/000338327